Detailed information of TA system TA054524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 111107..111764
• Replicon: plasmid pGPN1
• Accession: NZ_CP011540
• Organism: Klebsiella aerogenes strain G7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: EAG7_RS26455
• Protein ID: WP_000270043.1
• Coordinates: 111414..111764 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EAG7_RS26450
• Protein ID: WP_000124640.1
• Coordinates: 111107..111409 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAG7_RS26400	106732..107160	+	429	WP_000591074.1	hypothetical protein	-
EAG7_RS26405	107217..107576	+	360	WP_000422768.1	hypothetical protein	-
EAG7_RS26410	107576..108022	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
EAG7_RS26415	108019..108537	+	519	WP_000210758.1	nitrite reductase	-
EAG7_RS26420	108537..108767	+	231	WP_000972663.1	hypothetical protein	-
EAG7_RS26425	108754..109611	+	858	WP_001167032.1	hypothetical protein	-
EAG7_RS26435	109842..110369	+	528	WP_001236377.1	thermonuclease family protein	-
EAG7_RS26440	110427..110699	+	273	WP_001043047.1	HU family DNA-binding protein	-
EAG7_RS26445	110787..111080	+	294	WP_001239997.1	chromosome segregation protein ParM	-
EAG7_RS26450	111107..111409	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EAG7_RS26455	111414..111764	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EAG7_RS26460	111927..112475	+	549	WP_001061574.1	transcriptional regulator	-
EAG7_RS28890	112816..113010	+	195	WP_000343597.1	hypothetical protein	-
EAG7_RS28895	113021..113392	+	372	WP_000516916.1	hypothetical protein	-
EAG7_RS26480	113385..113855	+	471	WP_001281821.1	hypothetical protein	-
EAG7_RS26485	113870..114205	-	336	WP_000683476.1	hypothetical protein	-
EAG7_RS26490	114302..114790	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
EAG7_RS26495	114793..115290	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / blaTEM-24 / floR / sul2	-	1..174066	174066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T54524 WP_000270043.1 NZ_CP011540:c111764-111414 [Klebsiella aerogenes]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T54524 NZ_CP011540:c111764-111414 [Klebsiella aerogenes]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT54524 WP_000124640.1 NZ_CP011540:c111409-111107 [Klebsiella aerogenes]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT54524 NZ_CP011540:c111409-111107 [Klebsiella aerogenes]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9