Detailed information of TA system TA054446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 162681..163263
• Replicon: chromosome
• Accession: NZ_CP011514
• Organism: Mitsuaria sp. 7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ABE85_RS00740
• Protein ID: WP_067269190.1
• Coordinates: 162681..162992 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1A9HHF2
• Locus tag: ABE85_RS00745
• Protein ID: WP_067269192.1
• Coordinates: 162985..163263 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABE85_RS00710	158024..158452	-	429	WP_067269180.1	CBS domain-containing protein	-
ABE85_RS00715	158594..159124	-	531	WP_067269182.1	DUF962 domain-containing protein	-
ABE85_RS00720	159254..160033	+	780	WP_157521814.1	YceI family protein	-
ABE85_RS00725	160155..160733	-	579	WP_197507162.1	GNAT family N-acetyltransferase	-
ABE85_RS00730	160708..161322	-	615	WP_067269186.1	molybdenum cofactor guanylyltransferase	-
ABE85_RS00735	161322..162470	-	1149	WP_067269188.1	GTP 3',8-cyclase MoaA	-
ABE85_RS00740	162681..162992	+	312	WP_067269190.1	transcriptional regulator	Toxin
ABE85_RS00745	162985..163263	+	279	WP_067269192.1	helix-turn-helix domain-containing protein	Antitoxin
ABE85_RS00750	163336..164298	+	963	WP_067269193.1	carbohydrate kinase	-
ABE85_RS00755	164339..165541	-	1203	WP_067269195.1	multidrug effflux MFS transporter	-
ABE85_RS00760	165644..166255	-	612	WP_067269197.1	phenylacetic acid degradation protein PaaY	-
ABE85_RS00765	166419..167291	+	873	WP_067269199.1	enoyl-CoA hydratase/isomerase family protein	-
ABE85_RS00770	167315..167758	+	444	WP_157522935.1	hydroxyphenylacetyl-CoA thioesterase PaaI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-81)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11594.34 Da
• Isoelectric Point: 6.3366

>T54446 WP_067269190.1 NZ_CP011514:162681-162992 [Mitsuaria sp. 7]
MRTVAETPMFQKYARGVWTEAERVDFISWIALNPEAGDVIPGSGGCRKIRWTKAGRGKRGGVRVIYFNVEDEVIWLLIVY
SKAAYDSLPVAFLEELKQGVEDA

Nucleotide

• Length: 312 bp

>T54446 NZ_CP011514:162681-162992 [Mitsuaria sp. 7]
ATGCGAACCGTTGCCGAAACCCCGATGTTCCAGAAGTACGCGAGGGGCGTGTGGACCGAGGCGGAACGCGTGGACTTCAT
CAGCTGGATCGCACTGAACCCGGAAGCCGGAGACGTCATTCCGGGCAGCGGCGGTTGTCGCAAGATTCGCTGGACGAAGG
CGGGGCGCGGCAAGCGCGGTGGCGTTCGAGTGATCTATTTCAATGTCGAGGATGAGGTGATCTGGCTGTTGATCGTGTAT
TCGAAGGCTGCTTACGACAGTCTCCCCGTCGCCTTTCTGGAAGAGCTGAAGCAAGGAGTCGAAGATGCCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10230.82 Da
• Isoelectric Point: 10.4722

>AT54446 WP_067269192.1 NZ_CP011514:162985-163263 [Mitsuaria sp. 7]
MPKDKEKFQKDLLESIRQMKSGQAARVTRVEIPGAAEARACTGLSQTDFAGLLGVSKRTLQEWEQGRREPTGAARTLLRV
AMAHPEVLLELR

Nucleotide

• Length: 279 bp

>AT54446 NZ_CP011514:162985-163263 [Mitsuaria sp. 7]
ATGCCTAAGGACAAGGAGAAGTTCCAGAAGGACCTGCTGGAGTCGATCCGGCAGATGAAGTCCGGGCAGGCAGCCCGGGT
CACGCGCGTCGAGATTCCTGGAGCCGCAGAGGCTCGCGCCTGCACAGGCTTGTCCCAGACGGATTTCGCCGGTCTGCTGG
GGGTGTCGAAGCGGACGCTGCAGGAATGGGAGCAAGGACGGAGGGAACCGACCGGTGCTGCGAGGACGCTGCTACGCGTT
GCCATGGCGCACCCGGAGGTTCTGCTGGAGCTTCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A9HHF2