Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4203386..4204056 Replicon chromosome
Accession NZ_CP011510
Organism Mycobacterium tuberculosis strain Beijing

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag TM57_RS19590 Protein ID WP_003899954.1
Coordinates 4203712..4204056 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag TM57_RS19585 Protein ID WP_003899955.1
Coordinates 4203386..4203715 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM57_RS19560 4198801..4199835 + 1035 WP_003416786.1 IS30 family transposase -
TM57_RS19565 4200082..4200291 - 210 WP_003416778.1 hypothetical protein -
TM57_RS19570 4200459..4201724 + 1266 WP_003902423.1 hypothetical protein -
TM57_RS19575 4201884..4202504 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
TM57_RS19580 4202501..4202848 - 348 WP_003899956.1 DUF2384 domain-containing protein -
TM57_RS19585 4203386..4203715 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
TM57_RS19590 4203712..4204056 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TM57_RS19595 4204287..4204739 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
TM57_RS19600 4204742..4205176 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
TM57_RS19605 4205523..4206812 - 1290 WP_003416640.1 ATP-binding protein -
TM57_RS19610 4207100..4207393 - 294 WP_003416635.1 hypothetical protein -
TM57_RS19615 4207633..4208148 - 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
TM57_RS19620 4208123..4208983 - 861 WP_003416628.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T54430 WP_003899954.1 NZ_CP011510:c4204056-4203712 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T54430 NZ_CP011510:c4204056-4203712 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT54430 WP_003899955.1 NZ_CP011510:c4203715-4203386 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT54430 NZ_CP011510:c4203715-4203386 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References