Detailed information of TA system TA054352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 307354..307947
• Replicon: chromosome
• Accession: NZ_CP011504
• Organism: Burkholderia pyrrocinia strain DSM 10685

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ABD05_RS17710
• Protein ID: WP_047901478.1
• Coordinates: 307354..307632 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0G3WX68
• Locus tag: ABD05_RS17715
• Protein ID: WP_047901479.1
• Coordinates: 307645..307947 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABD05_RS17690	302898..304331	-	1434	WP_047901475.1	aldehyde dehydrogenase family protein	-
ABD05_RS17695	304361..306001	-	1641	WP_047901476.1	acetolactate synthase large subunit	-
ABD05_RS17700	306331..306669	+	339	WP_047901477.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABD05_RS17705	306673..306972	+	300	WP_039344756.1	putative addiction module antidote protein	-
ABD05_RS17710	307354..307632	+	279	WP_047901478.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABD05_RS17715	307645..307947	+	303	WP_047901479.1	HigA family addiction module antidote protein	Antitoxin
ABD05_RS17720	307981..309267	-	1287	WP_047901480.1	DUF445 domain-containing protein	-
ABD05_RS17725	309479..310699	-	1221	WP_047901481.1	FAD-dependent oxidoreductase	-
ABD05_RS17730	310689..311015	-	327	WP_034185113.1	non-heme iron oxygenase ferredoxin subunit	-
ABD05_RS17735	311033..311509	-	477	WP_014900133.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
ABD05_RS17740	311524..312795	-	1272	WP_047903620.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10657.13 Da
• Isoelectric Point: 8.5441

>T54352 WP_047901478.1 NZ_CP011504:307354-307632 [Burkholderia pyrrocinia]
MIRSFRCKDTQLLFAGESPKRFRTIETVATRKLQMVAAAKELRDLRAPPGNRLEALQGDRGGQHSIRINEQWRICFEWSD
DGPTQVEIVDYH

Nucleotide

• Length: 279 bp

>T54352 NZ_CP011504:307354-307632 [Burkholderia pyrrocinia]
ATGATCAGATCGTTCCGATGCAAGGATACCCAGTTGCTGTTTGCTGGAGAATCGCCCAAGCGCTTCCGCACGATCGAAAC
CGTTGCCACCCGGAAACTGCAAATGGTCGCCGCTGCGAAAGAACTGCGCGATTTGCGGGCGCCTCCCGGCAACCGGCTCG
AGGCGTTGCAAGGCGATCGGGGAGGCCAGCACAGCATCCGCATCAACGAGCAATGGCGGATCTGTTTCGAATGGAGCGAC
GATGGCCCCACCCAGGTCGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11556.34 Da
• Isoelectric Point: 8.5562

>AT54352 WP_047901479.1 NZ_CP011504:307645-307947 [Burkholderia pyrrocinia]
MLKNGMRPIHPGEVLREEFLVPLEMSVNALALALNVPATRLHEIVKERRGITADTAYRLARYFDTSPEFWLNLQAAYDLK
TLPTRSEIDRKVEPRERTHA

Nucleotide

• Length: 303 bp

>AT54352 NZ_CP011504:307645-307947 [Burkholderia pyrrocinia]
ATGCTCAAGAATGGAATGCGCCCGATTCATCCGGGCGAGGTCCTGCGGGAAGAGTTCCTCGTTCCGCTGGAAATGAGCGT
CAACGCACTGGCGCTCGCGTTGAACGTGCCGGCGACACGCTTGCACGAAATCGTCAAGGAACGGCGCGGCATCACGGCCG
ATACGGCTTACCGGCTTGCGCGCTACTTCGATACGTCGCCGGAATTCTGGTTGAACCTGCAAGCCGCTTACGATCTCAAG
ACACTGCCGACGCGCAGCGAGATCGACCGCAAGGTCGAACCGCGCGAGCGCACGCACGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0G3WX68