Detailed information of TA system TA054345

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66247..66668
• Replicon: plasmid F
• Accession: NZ_CP011496
• Organism: Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: AA953_RS23620
• Protein ID: WP_001302184.1
• Coordinates: 66510..66668 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66247..66445 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA953_RS23590	62177..62716	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
AA953_RS23595	62773..63006	+	234	WP_000005990.1	DUF905 family protein	-
AA953_RS23600	63072..65030	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
AA953_RS23605	65085..65519	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
AA953_RS23610	65516..66235	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	66247..66445	+	199	NuclAT_0	-	Antitoxin
-	66247..66445	+	199	NuclAT_0	-	Antitoxin
-	66247..66445	+	199	NuclAT_0	-	Antitoxin
-	66247..66445	+	199	NuclAT_0	-	Antitoxin
AA953_RS23620	66510..66668	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
AA953_RS26905	66906..67265	-	360	Protein_77	hypothetical protein	-
AA953_RS26910	67335..67541	+	207	WP_000547965.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67545	67545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T54345 WP_001302184.1 NZ_CP011496:66510-66668 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T54345 NZ_CP011496:66510-66668 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT54345 NZ_CP011496:66247-66445 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin