Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3349094..3349596 Replicon chromosome
Accession NZ_CP011495
Organism Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AA953_RS16815 Protein ID WP_000767829.1
Coordinates 3349094..3349348 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AA953_RS16820 Protein ID WP_001259255.1
Coordinates 3349345..3349596 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA953_RS16790 3344109..3344336 - 228 WP_000234896.1 sulfurtransferase TusA -
AA953_RS16795 3344350..3345408 - 1059 WP_000492339.1 transport protein YeeE -
AA953_RS16800 3345587..3346945 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AA953_RS26590 3346935..3346997 - 63 WP_010723108.1 membrane protein YoeI -
AA953_RS16805 3347212..3348141 - 930 WP_000803351.1 LysR family transcriptional regulator -
AA953_RS16810 3348187..3349011 - 825 WP_000754737.1 SDR family oxidoreductase -
AA953_RS16815 3349094..3349348 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AA953_RS16820 3349345..3349596 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AA953_RS25155 3349879..3349929 + 51 WP_001364200.1 his operon leader peptide -
AA953_RS16825 3350075..3350974 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AA953_RS16830 3350980..3352284 + 1305 WP_000009594.1 histidinol dehydrogenase -
AA953_RS16835 3352281..3353351 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
AA953_RS16840 3353351..3354418 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T54335 WP_000767829.1 NZ_CP011495:c3349348-3349094 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T54335 NZ_CP011495:c3349348-3349094 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT54335 WP_001259255.1 NZ_CP011495:c3349596-3349345 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT54335 NZ_CP011495:c3349596-3349345 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References