Detailed information of TA system TA054310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1470525..1471219
• Replicon: chromosome
• Accession: NZ_CP011495
• Organism: Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AA953_RS07165
• Protein ID: WP_001263489.1
• Coordinates: 1470821..1471219 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AA953_RS07160
• Protein ID: WP_000554758.1
• Coordinates: 1470525..1470818 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA953_RS07140	1466157..1466654	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
AA953_RS07145	1466878..1468590	-	1713	Protein_1331	flagellar biosynthesis protein FlhA	-
AA953_RS07150	1468562..1469347	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AA953_RS07155	1469418..1470473	+	1056	WP_001226164.1	DNA polymerase IV	-
AA953_RS07160	1470525..1470818	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AA953_RS07165	1470821..1471219	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AA953_RS07170	1471229..1471681	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AA953_RS07175	1471999..1472205	+	207	Protein_1337	RtcB family protein	-
AA953_RS07180	1472201..1472722	+	522	Protein_1338	peptide chain release factor H	-
AA953_RS07185	1472779..1474236	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AA953_RS07190	1474497..1474955	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1475551..1475631	+	81	NuclAT_12	-	-
-	1475551..1475631	+	81	NuclAT_12	-	-
-	1475551..1475631	+	81	NuclAT_12	-	-
-	1475551..1475631	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T54310 WP_001263489.1 NZ_CP011495:1470821-1471219 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T54310 NZ_CP011495:1470821-1471219 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT54310 WP_000554758.1 NZ_CP011495:1470525-1470818 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT54310 NZ_CP011495:1470525-1470818 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3