Detailed information of TA system TA054293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1231484..1232072
• Replicon: chromosome
• Accession: NZ_CP011483
• Organism: Helicobacter pylori strain DU15

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: -
• Locus tag: EE66_RS06025
• Protein ID: WP_064431335.1
• Coordinates: 1231791..1232072 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: T5CP67
• Locus tag: EE66_RS06020
• Protein ID: WP_000065290.1
• Coordinates: 1231484..1231798 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EE66_RS06000	1226995..1227651	+	657	WP_064431332.1	ParA family protein	-
EE66_RS06005	1227736..1228017	+	282	WP_000394649.1	hypothetical protein	-
EE66_RS06010	1228061..1229245	+	1185	WP_064431333.1	hypothetical protein	-
EE66_RS06015	1229270..1231180	-	1911	WP_064431334.1	relaxase/mobilization nuclease domain-containing protein	-
EE66_RS06020	1231484..1231798	+	315	WP_000065290.1	hypothetical protein	Antitoxin
EE66_RS06025	1231791..1232072	+	282	WP_064431335.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
EE66_RS06030	1232356..1234083	-	1728	WP_064431336.1	type IV secretory system conjugative DNA transfer family protein	-
EE66_RS06035	1234084..1234641	-	558	WP_064431337.1	hypothetical protein	-
EE66_RS06040	1234642..1234932	-	291	WP_000159825.1	hypothetical protein	-
EE66_RS06045	1234929..1235384	-	456	WP_000795419.1	hypothetical protein	-
EE66_RS06050	1235381..1236323	-	943	Protein_1162	Flp pilus assembly complex ATPase component TadA	-
EE66_RS06055	1236313..1236624	-	312	WP_064431338.1	hypothetical protein	-
EE66_RS06060	1236621..1236884	-	264	WP_064431339.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tlpA / tlpB / flaB	1158332..1264058	105726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10957.89 Da
• Isoelectric Point: 10.1839

>T54293 WP_064431335.1 NZ_CP011483:1231791-1232072 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSVVDRLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEVKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T54293 NZ_CP011483:1231791-1232072 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTACAAGCGTTGTTGATCGCCTCAAAGAGCAAAAACCACTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAGTCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12149.63 Da
• Isoelectric Point: 5.6753

>AT54293 WP_000065290.1 NZ_CP011483:1231484-1231798 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT54293 NZ_CP011483:1231484-1231798 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTATTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Antitoxin

• AlphaFold DB: T5CP67