Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 39452..40135 Replicon chromosome
Accession NZ_CP011427
Organism Pantoea vagans strain ND02

Toxin (Protein)


Gene name higB Uniprot ID A0A0B1R082
Locus tag LK04_RS00180 Protein ID WP_039337348.1
Coordinates 39452..39760 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0B1R1V4
Locus tag LK04_RS00185 Protein ID WP_039337346.1
Coordinates 39803..40135 (+) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK04_RS00160 34643..35137 + 495 WP_039333095.1 GNAT family N-acetyltransferase -
LK04_RS00165 35223..36137 - 915 WP_039333098.1 sugar ABC transporter substrate-binding protein -
LK04_RS00170 36572..37966 + 1395 WP_059109754.1 MFS transporter -
LK04_RS00175 37947..38906 + 960 WP_039337350.1 2-hydroxyacid dehydrogenase -
LK04_RS00180 39452..39760 + 309 WP_039337348.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LK04_RS00185 39803..40135 + 333 WP_039337346.1 HigA family addiction module antidote protein Antitoxin
LK04_RS00190 40322..41485 + 1164 WP_039337345.1 HD-GYP domain-containing protein -
LK04_RS00195 41594..41683 + 90 WP_100701897.1 K(+)-transporting ATPase subunit F -
LK04_RS00200 41683..43362 + 1680 WP_039337343.1 potassium-transporting ATPase subunit KdpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 12154.84 Da        Isoelectric Point: 9.7731

>T54242 WP_039337348.1 NZ_CP011427:39452-39760 [Pantoea vagans]
MTGAIKHFRDDWLQQFFIYATPHRHLPKSIESALARKLDIINAAKTYHDLRSPPGNRLETLKPPLQGYYAIRVNEQYRLI
FRWINGEASDLYLDPHSYRHYQ

Download         Length: 309 bp

>T54242 NZ_CP011427:39452-39760 [Pantoea vagans]
ATGACGGGTGCAATTAAACATTTTAGAGATGATTGGCTTCAGCAATTTTTTATCTATGCCACACCGCACAGACATCTCCC
CAAGTCGATTGAATCCGCACTGGCGCGCAAACTCGACATTATCAATGCCGCCAAAACGTATCATGATCTGCGATCGCCAC
CCGGGAACCGTCTGGAAACTCTAAAACCCCCCTTGCAGGGTTATTACGCTATCCGCGTAAATGAGCAATATCGGCTTATT
TTTCGTTGGATAAATGGAGAAGCCAGCGATCTTTATCTCGATCCGCATAGTTACAGACACTACCAATAA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12724.41 Da        Isoelectric Point: 6.4679

>AT54242 WP_039337346.1 NZ_CP011427:39803-40135 [Pantoea vagans]
MNQAKRKPTSVGDVLQYEFLEPLHLKVNDLAEMLNVHRNTASALVNNNRKMTTEMAYRLAAVFATSVDFWLNLQRYQDQW
EVENDARTQEELSRVTPLNTFLAQRGIQVA

Download         Length: 333 bp

>AT54242 NZ_CP011427:39803-40135 [Pantoea vagans]
ATGAATCAGGCAAAGCGTAAGCCCACCAGTGTAGGTGATGTGCTGCAATATGAGTTCCTCGAACCCCTCCATCTTAAAGT
CAATGACTTGGCTGAGATGCTGAATGTACACAGAAATACGGCCAGCGCATTGGTCAATAACAACCGCAAAATGACCACTG
AGATGGCGTACCGTTTAGCGGCGGTTTTTGCGACCTCTGTCGATTTTTGGCTTAACCTGCAACGCTATCAGGACCAGTGG
GAAGTGGAAAATGATGCCCGCACCCAAGAAGAGTTAAGTCGTGTCACGCCGTTAAATACCTTTTTAGCGCAGCGTGGGAT
TCAGGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B1R082

Antitoxin

Source ID Structure
AlphaFold DB A0A0B1R1V4

References