Detailed information of TA system TA054230

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45121..45374
• Replicon: plasmid pCFSAN029787_02
• Accession: NZ_CP011418
• Organism: Escherichia coli strain CFSAN029787

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: AA102_RS27300
• Protein ID: WP_001336447.1
• Coordinates: 45121..45270 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	45318..45374 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA102_RS27045	40597..41367	+	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-
AA102_RS27050	41397..41644	+	248	Protein_46	hypothetical protein	-
AA102_RS31945	41794..41892	-	99	WP_137491101.1	transposase	-
AA102_RS27060	42085..42393	+	309	Protein_48	transposase	-
AA102_RS31655	42508..42720	-	213	WP_001336932.1	hypothetical protein	-
AA102_RS27065	43421..44278	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
AA102_RS29460	44271..44345	-	75	WP_001365705.1	RepA leader peptide Tap	-
AA102_RS27075	44579..44836	-	258	WP_000084404.1	replication regulatory protein RepA	-
AA102_RS27300	45121..45270	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	45318..45374	+	57	NuclAT_0	-	Antitoxin
-	45318..45374	+	57	NuclAT_0	-	Antitoxin
-	45318..45374	+	57	NuclAT_0	-	Antitoxin
-	45318..45374	+	57	NuclAT_0	-	Antitoxin
AA102_RS27080	45469..45906	-	438	WP_000872609.1	hypothetical protein	-
AA102_RS27085	46080..46442	-	363	WP_046881847.1	hypothetical protein	-
AA102_RS27090	46545..47006	-	462	WP_000760080.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	-	1..47606	47606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T54230 WP_001336447.1 NZ_CP011418:c45270-45121 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T54230 NZ_CP011418:c45270-45121 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT54230 NZ_CP011418:45318-45374 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin