Detailed information of TA system TA054208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2308589..2309191
• Replicon: chromosome
• Accession: NZ_CP011416
• Organism: Escherichia coli strain CFSAN029787

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AA102_RS11715
• Protein ID: WP_000897305.1
• Coordinates: 2308589..2308900 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AA102_RS11720
• Protein ID: WP_000356397.1
• Coordinates: 2308901..2309191 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA102_RS11685	2303619..2304404	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AA102_RS11690	2304503..2305102	+	600	WP_001295269.1	glucose-1-phosphatase	-
AA102_RS11695	2305096..2305968	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AA102_RS11700	2305965..2306402	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AA102_RS11705	2306447..2307388	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AA102_RS11710	2307452..2308360	-	909	WP_001299484.1	alpha/beta hydrolase	-
AA102_RS11715	2308589..2308900	+	312	WP_000897305.1	hypothetical protein	Toxin
AA102_RS11720	2308901..2309191	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AA102_RS11725	2309796..2310014	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AA102_RS11730	2310232..2310474	+	243	WP_001086388.1	hypothetical protein	-
AA102_RS11740	2310703..2311683	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
AA102_RS11745	2312083..2313012	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AA102_RS11750	2313009..2313644	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2310703..2311683	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T54208 WP_000897305.1 NZ_CP011416:2308589-2308900 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T54208 NZ_CP011416:2308589-2308900 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT54208 WP_000356397.1 NZ_CP011416:2308901-2309191 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT54208 NZ_CP011416:2308901-2309191 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7