Detailed information of TA system TA054197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3578599..3579202
• Replicon: chromosome
• Accession: NZ_CP011409
• Organism: Herbaspirillum hiltneri N3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: F506_RS16350
• Protein ID: WP_053199151.1
• Coordinates: 3578924..3579202 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F506_RS16345
• Protein ID: WP_053199149.1
• Coordinates: 3578599..3578910 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F506_RS16335	3574638..3576950	-	2313	WP_053199145.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
F506_RS16340	3577085..3578005	+	921	WP_053199147.1	LysR family transcriptional regulator	-
F506_RS22740	3578272..3578568	+	297	WP_083457962.1	hypothetical protein	-
F506_RS16345	3578599..3578910	-	312	WP_053199149.1	HigA family addiction module antidote protein	Antitoxin
F506_RS16350	3578924..3579202	-	279	WP_053199151.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F506_RS16355	3579455..3581146	-	1692	WP_053199153.1	sensor domain-containing diguanylate cyclase	-
F506_RS16360	3581450..3584041	-	2592	WP_053199155.1	ATP-dependent chaperone ClpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-99)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10610.02 Da
• Isoelectric Point: 7.8607

>T54197 WP_053199151.1 NZ_CP011409:c3579202-3578924 [Herbaspirillum hiltneri N3]
MILSFQCKDTESFYQGQSVARFANFRNTAERKLQMLDSAVTLDFLRSPPGNRLEALIGNRAGQHSIRINDQFRICFVWTD
KGPTQVEIVDYH

Nucleotide

• Length: 279 bp

>T54197 NZ_CP011409:c3579202-3578924 [Herbaspirillum hiltneri N3]
ATGATACTTAGCTTCCAATGCAAAGATACCGAGTCCTTTTACCAAGGGCAGAGCGTTGCGAGGTTTGCAAATTTCAGAAA
CACAGCTGAACGCAAACTGCAAATGCTTGACTCCGCAGTAACGTTGGACTTTCTCCGTTCTCCACCGGGAAATCGGTTGG
AGGCGCTCATCGGCAATCGTGCCGGGCAGCACAGTATCAGGATCAACGATCAATTCCGTATCTGTTTCGTGTGGACTGAT
AAGGGACCAACTCAAGTCGAGATCGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11505.35 Da
• Isoelectric Point: 5.7203

>AT54197 WP_053199149.1 NZ_CP011409:c3578910-3578599 [Herbaspirillum hiltneri N3]
MTKLINRMRPVHPGEILKEDFLAPAELSANALAIALHVPATRIGEIINERRGITPDTALRLARFFGGDAQSWLNLQLAYD
LKITEMEVGERIEREIQPMAQAA

Nucleotide

• Length: 312 bp

>AT54197 NZ_CP011409:c3578910-3578599 [Herbaspirillum hiltneri N3]
ATGACTAAATTAATTAATCGTATGCGCCCGGTTCATCCGGGCGAAATTCTTAAAGAAGACTTTCTTGCTCCTGCAGAGTT
GAGCGCCAATGCATTGGCTATTGCCTTGCACGTCCCTGCTACGCGTATTGGGGAAATCATCAATGAACGCCGAGGTATTA
CTCCGGACACCGCCTTGCGACTCGCGCGCTTCTTTGGCGGCGACGCCCAATCCTGGCTGAATCTGCAGCTCGCTTACGAT
CTCAAAATAACTGAGATGGAAGTGGGCGAACGGATCGAACGAGAAATTCAGCCTATGGCGCAGGCTGCATAA