Detailed information of TA system TA054170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2185228..2185832
• Replicon: chromosome
• Accession: NZ_CP011367
• Organism: Thioalkalivibrio versutus strain D301

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0G3G3C3
• Locus tag: TVD_RS10085
• Protein ID: WP_047251536.1
• Coordinates: 2185228..2185512 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TVD_RS10090
• Protein ID: WP_018649772.1
• Coordinates: 2185533..2185832 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVD_RS10070	2181483..2182508	-	1026	WP_018938748.1	efflux RND transporter periplasmic adaptor subunit	-
TVD_RS10075	2182795..2183136	+	342	WP_018649775.1	hypothetical protein	-
TVD_RS10080	2183171..2184883	+	1713	WP_047251535.1	proline--tRNA ligase	-
TVD_RS10085	2185228..2185512	+	285	WP_047251536.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVD_RS10090	2185533..2185832	+	300	WP_018649772.1	HigA family addiction module antidote protein	Antitoxin
TVD_RS10095	2185912..2187774	+	1863	WP_047251537.1	SLC13 family permease	-
TVD_RS10100	2187843..2188457	+	615	WP_047251538.1	hypothetical protein	-
TVD_RS10105	2188470..2188760	-	291	WP_018649769.1	acylphosphatase	-
TVD_RS10110	2188784..2189140	+	357	WP_018649768.1	arsenate reductase (glutaredoxin)	-
TVD_RS10115	2189137..2189748	+	612	WP_018649767.1	NAD(P)H:quinone oxidoreductase	-
TVD_RS10120	2189745..2190101	+	357	WP_018143910.1	DUF2069 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10941.51 Da
• Isoelectric Point: 10.1008

>T54170 WP_047251536.1 NZ_CP011367:2185228-2185512 [Thioalkalivibrio versutus]
MLSVSLGKEAERVAQGESCRRIPADIVRRARMRLARIHAATQLDDLKVPPSHHLERLKGNRAGQYSIRINAQWRICFEWH
DGHAYNVEIVDYHR

Nucleotide

• Length: 285 bp

>T54170 NZ_CP011367:2185228-2185512 [Thioalkalivibrio versutus]
TTGCTCAGCGTCTCCCTCGGCAAGGAAGCCGAACGCGTGGCGCAGGGCGAGTCATGTCGCCGAATACCGGCCGATATCGT
ACGCCGCGCGCGGATGCGGCTGGCCCGCATCCATGCCGCCACCCAACTGGACGACCTGAAGGTTCCGCCGTCGCACCACC
TGGAACGGCTCAAGGGAAACCGGGCGGGCCAGTACAGCATCCGCATCAACGCGCAATGGCGGATCTGTTTTGAATGGCAC
GACGGTCATGCATACAACGTCGAGATCGTGGACTATCACCGATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11315.70 Da
• Isoelectric Point: 6.4851

>AT54170 WP_018649772.1 NZ_CP011367:2185533-2185832 [Thioalkalivibrio versutus]
MNHENSHNPIHPGDHLAEFLEETGISQYRLALDISVPPRRINEIVRGKRAITADTALRLGRYFGTSPEFWMNLQDHYELA
CAQQALGNRLDQEVRPHVA

Nucleotide

• Length: 300 bp

>AT54170 NZ_CP011367:2185533-2185832 [Thioalkalivibrio versutus]
ATGAATCATGAAAATTCGCATAACCCGATCCATCCAGGGGACCACTTGGCCGAATTCCTGGAAGAGACAGGGATCAGCCA
ATACCGCCTGGCGCTGGATATCAGCGTCCCACCCCGCCGGATCAACGAGATCGTTCGCGGCAAGCGCGCCATCACCGCCG
ACACAGCCCTTCGTCTGGGCCGATACTTCGGCACCTCGCCCGAGTTCTGGATGAATCTTCAGGACCACTACGAGCTGGCG
TGCGCTCAACAGGCGCTGGGCAATCGGCTCGACCAGGAAGTTCGCCCGCACGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0G3G3C3