Detailed information of TA system TA054168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 317484..318086
• Replicon: chromosome
• Accession: NZ_CP011367
• Organism: Thioalkalivibrio versutus strain D301

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: TVD_RS01465
• Protein ID: WP_082119055.1
• Coordinates: 317484..317762 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TVD_RS01470
• Protein ID: WP_018144608.1
• Coordinates: 317787..318086 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVD_RS01435	312641..313711	-	1071	WP_047251878.1	PilW family protein	-
TVD_RS01440	313721..314173	-	453	WP_047250616.1	type IV pilus modification protein PilV	-
TVD_RS13890	314219..314713	-	495	WP_082119054.1	GspH/FimT family pseudopilin	-
TVD_RS01445	314855..315379	-	525	WP_047250617.1	GspH/FimT family pseudopilin	-
TVD_RS01450	315685..316551	+	867	WP_047250618.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
TVD_RS01455	316658..317038	-	381	WP_047250619.1	type II toxin-antitoxin system VapC family toxin	-
TVD_RS01460	317038..317280	-	243	WP_047250620.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
TVD_RS01465	317484..317762	+	279	WP_082119055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVD_RS01470	317787..318086	+	300	WP_018144608.1	HigA family addiction module antidote protein	Antitoxin
TVD_RS01475	318145..318684	-	540	WP_047250621.1	protein disulfide oxidoreductase	-
TVD_RS01480	318681..319112	-	432	WP_047250622.1	hypothetical protein	-
TVD_RS01485	319247..320329	+	1083	WP_047250623.1	quinolinate synthase NadA	-
TVD_RS01490	320373..320684	+	312	WP_018144604.1	nucleotidyltransferase family protein	-
TVD_RS01495	320689..321030	+	342	WP_047251879.1	DUF86 domain-containing protein	-
TVD_RS01500	321107..321313	-	207	WP_047250624.1	hypothetical protein	-
TVD_RS01505	321412..321768	+	357	WP_047250625.1	hypothetical protein	-
TVD_RS01510	322072..322602	-	531	WP_018168330.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	307595..318086	10491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10784.18 Da
• Isoelectric Point: 9.4239

>T54168 WP_082119055.1 NZ_CP011367:317484-317762 [Thioalkalivibrio versutus]
MIRSFRCRETRALFETGRSRRWSSVRAIAERKLIQLQAAETLQDLRSPPGNRLEALSGDRVGQHSIRINAQWRICFRWTD
EGAEDVEIVDYH

Nucleotide

• Length: 279 bp

>T54168 NZ_CP011367:317484-317762 [Thioalkalivibrio versutus]
ATGATCAGAAGCTTTCGGTGTAGGGAAACCCGGGCGCTGTTCGAAACGGGCAGAAGTCGACGTTGGTCGAGCGTCCGAGC
GATCGCGGAACGCAAGCTGATTCAGCTTCAAGCGGCGGAGACGCTGCAGGATTTGCGGTCACCGCCGGGGAACCGGCTGG
AGGCCCTGTCTGGTGACCGCGTGGGACAGCACAGCATTCGCATCAATGCCCAGTGGCGCATTTGTTTCCGGTGGACGGAC
GAGGGTGCCGAGGACGTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11262.91 Da
• Isoelectric Point: 8.6801

>AT54168 WP_018144608.1 NZ_CP011367:317787-318086 [Thioalkalivibrio versutus]
MTTNGMRPIHPGEVLREEFLAPLGLSVNALAGALHVPTTRMNDIVRERRGVSADTAMRLARYFGTSERFWLNLQVEYELR
RAQIELADRVRSEIEPRAA

Nucleotide

• Length: 300 bp

>AT54168 NZ_CP011367:317787-318086 [Thioalkalivibrio versutus]
ATGACTACAAACGGTATGCGGCCCATCCATCCAGGCGAGGTCCTGCGCGAGGAGTTTCTTGCTCCGTTAGGGCTTAGTGT
GAACGCGCTTGCCGGGGCATTGCATGTACCGACGACGCGGATGAATGACATTGTGCGCGAGCGCCGGGGCGTCTCCGCCG
ACACGGCCATGAGGCTGGCGCGTTATTTCGGTACTTCCGAGCGTTTCTGGCTGAACCTGCAGGTTGAATACGAATTGCGG
CGTGCCCAGATCGAACTCGCCGACCGCGTGCGTAGCGAGATCGAACCCCGCGCCGCGTGA