Detailed information of TA system TA054167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 77224..77844
• Replicon: chromosome
• Accession: NZ_CP011367
• Organism: Thioalkalivibrio versutus strain D301

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0G3FY48
• Locus tag: TVD_RS00380
• Protein ID: WP_018175555.1
• Coordinates: 77551..77844 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TVD_RS00375
• Protein ID: WP_024326782.1
• Coordinates: 77224..77541 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TVD_RS00360	73147..74364	+	1218	WP_047250496.1	glycosyltransferase, exosortase A system-associated	-
TVD_RS00365	74354..75613	+	1260	WP_047250497.1	glycosyltransferase	-
TVD_RS00370	75616..76989	+	1374	WP_047250498.1	phenylacetate--CoA ligase family protein	-
TVD_RS00375	77224..77541	-	318	WP_024326782.1	HigA family addiction module antidote protein	Antitoxin
TVD_RS00380	77551..77844	-	294	WP_018175555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TVD_RS00385	78121..79923	-	1803	WP_082119048.1	asparagine synthase	-
TVD_RS00390	80282..80533	+	252	WP_018169304.1	acyl carrier protein	-
TVD_RS00395	80543..81346	+	804	WP_047250500.1	hydrolase 2, exosortase A system-associated	-
TVD_RS00400	81343..82230	+	888	WP_047250501.1	hydrolase 1, exosortase A system-associated	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11059.59 Da
• Isoelectric Point: 6.9255

>T54167 WP_018175555.1 NZ_CP011367:c77844-77551 [Thioalkalivibrio versutus]
MIVCFKDRGTEDIFNGLSSKAARKVCPQSVWRVAARKLELLDSAMNVEELRVPPGNRLEALAGERKGQYSIRINEQYRIC
FAWTDSGPDQVEITDYH

Nucleotide

• Length: 294 bp

>T54167 NZ_CP011367:c77844-77551 [Thioalkalivibrio versutus]
ATGATTGTATGCTTCAAGGATCGAGGCACTGAGGATATCTTCAACGGGCTTTCGTCGAAGGCTGCTCGCAAGGTCTGCCC
ACAATCTGTATGGCGGGTTGCCGCAAGAAAGCTGGAATTGCTGGATTCGGCCATGAACGTTGAGGAGCTGAGAGTCCCAC
CGGGTAACCGGCTGGAGGCCCTGGCGGGAGAGCGAAAAGGCCAATACAGCATTCGAATCAACGAGCAATATCGGATCTGC
TTCGCCTGGACAGACTCCGGGCCCGACCAGGTCGAAATCACGGATTACCACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12387.28 Da
• Isoelectric Point: 10.3486

>AT54167 WP_024326782.1 NZ_CP011367:c77541-77224 [Thioalkalivibrio versutus]
MVRIPTHRQPTHPGEMLAKEFLEPMHISQRQLADALHVPYQRINELVNQKRGITPSTSLRLARFFGMSPDFWLNLQVRWD
LFKAEQSEQADLKQIREFRSCKQTA

Nucleotide

• Length: 318 bp

>AT54167 NZ_CP011367:c77541-77224 [Thioalkalivibrio versutus]
ATGGTCCGCATCCCTACCCACCGACAGCCGACCCACCCCGGTGAAATGCTGGCGAAAGAGTTCCTGGAGCCGATGCATAT
CTCCCAGCGCCAGTTGGCGGACGCGCTCCATGTTCCGTATCAGCGCATCAACGAGCTCGTGAACCAGAAGCGCGGCATTA
CGCCAAGCACGTCGCTGCGTCTAGCACGCTTTTTCGGCATGTCGCCCGATTTCTGGTTGAACCTTCAGGTACGCTGGGAT
TTGTTCAAGGCGGAGCAATCGGAACAGGCGGATCTGAAACAGATCCGGGAATTCCGTTCGTGTAAGCAAACTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0G3FY48