Detailed information of TA system TA054150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2644697..2645298
• Replicon: chromosome
• Accession: NZ_CP011360
• Organism: Bradyrhizobium diazoefficiens USDA 110

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: AAV28_RS11570
• Protein ID: WP_011085796.1
• Coordinates: 2645017..2645298 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AAV28_RS11565
• Protein ID: WP_011085795.1
• Coordinates: 2644697..2645005 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAV28_RS11550	2639943..2642531	+	2589	WP_011085792.1	MMPL family transporter	-
AAV28_RS11555	2642499..2643155	-	657	WP_038965759.1	DUF2147 domain-containing protein	-
AAV28_RS11560	2643297..2644688	+	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
AAV28_RS11565	2644697..2645005	-	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
AAV28_RS11570	2645017..2645298	-	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AAV28_RS11575	2645370..2646137	-	768	WP_011085797.1	anti-sigma factor	-
AAV28_RS11580	2646134..2646682	-	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
AAV28_RS11585	2646810..2647145	-	336	WP_011085799.1	cupredoxin family copper-binding protein	-
AAV28_RS11590	2647171..2648115	-	945	WP_011085800.1	metallophosphoesterase	-
AAV28_RS11595	2648295..2649161	+	867	WP_011085801.1	helix-turn-helix transcriptional regulator	-
AAV28_RS11600	2649184..2649507	-	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
AAV28_RS11605	2649553..2649915	-	363	WP_011085803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T54150 WP_011085796.1 NZ_CP011360:c2645298-2645017 [Bradyrhizobium diazoefficiens USDA 110]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T54150 NZ_CP011360:c2645298-2645017 [Bradyrhizobium diazoefficiens USDA 110]
GTGATCAGAACGTTTCGGGACAAAACGACTGAGGCAGTCTTCGACGGAGAAAGTCCCAAAGGCTTTCCAGCCGATCTCGT
GAAGGTTGCACGCCGGAAACTGCGTTATCTGCACGCTGCCGGCGAGCTCGGCGATCTAAGGGCGCCGCCCGGCAACCGTC
TGGAGGCCCTGAGCGGCAATCGCAAGGGACAGCACTCGATCAGGATCAATGATCAGTTCCGAGTGTGCTTCATCTGGACA
CCGCAAGGGCCCGTCGAGGTCGAGATTGTGGACTATCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT54150 WP_011085795.1 NZ_CP011360:c2645005-2644697 [Bradyrhizobium diazoefficiens USDA 110]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT54150 NZ_CP011360:c2645005-2644697 [Bradyrhizobium diazoefficiens USDA 110]
ATGGCCAAAAAACTCAAGCCCATGCACCCCGGTGAGGTTCTACGGGAGGAGTTTCTAGTCCCACTGAACATGTCCGCTGG
CGCGCTCGCCAAGACGTGCGGCTTGCCGCGCACGCGCATCGAGCGGATCGCGAATGAGCAGACCGGAATCACCGCCGACA
CCGCGCTTCGACTGGCCAAGGCGCTCGGCACAACGGCCGAACTCTGGCTCAATCTTCAGAATGATTATGACATCCAGATC
GCGAAGCGCGATCTCGGCAAGGCACTTGACCGCATCGAGACCGTGAACAGGCCGCAAGCGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2