Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2069369..2069871 Replicon chromosome
Accession NZ_CP011343
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag U069_RS10420 Protein ID WP_000767829.1
Coordinates 2069369..2069623 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag U069_RS10425 Protein ID WP_001259255.1
Coordinates 2069620..2069871 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U069_RS10395 2064384..2064611 - 228 WP_000234896.1 sulfurtransferase TusA -
U069_RS10400 2064625..2065683 - 1059 WP_000492339.1 transport protein YeeE -
U069_RS10405 2065862..2067220 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
U069_RS25455 2067210..2067272 - 63 WP_010723108.1 membrane protein YoeI -
U069_RS10410 2067487..2068416 - 930 WP_000803351.1 LysR family transcriptional regulator -
U069_RS10415 2068462..2069286 - 825 WP_000754737.1 SDR family oxidoreductase -
U069_RS10420 2069369..2069623 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
U069_RS10425 2069620..2069871 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
U069_RS23765 2070154..2070204 + 51 WP_001364200.1 his operon leader peptide -
U069_RS10430 2070350..2071249 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
U069_RS10435 2071255..2072559 + 1305 WP_000009594.1 histidinol dehydrogenase -
U069_RS10440 2072556..2073626 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
U069_RS10445 2073626..2074693 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T54109 WP_000767829.1 NZ_CP011343:c2069623-2069369 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T54109 NZ_CP011343:c2069623-2069369 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT54109 WP_001259255.1 NZ_CP011343:c2069871-2069620 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT54109 NZ_CP011343:c2069871-2069620 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References