Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2070065..2070567 Replicon chromosome
Accession NZ_CP011342
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag U068_RS10450 Protein ID WP_000767829.1
Coordinates 2070065..2070319 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag U068_RS10455 Protein ID WP_001259255.1
Coordinates 2070316..2070567 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U068_RS10425 2065080..2065307 - 228 WP_000234896.1 sulfurtransferase TusA -
U068_RS10430 2065321..2066379 - 1059 WP_000492339.1 transport protein YeeE -
U068_RS10435 2066558..2067916 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
U068_RS25400 2067906..2067968 - 63 WP_010723108.1 membrane protein YoeI -
U068_RS10440 2068183..2069112 - 930 WP_000803351.1 LysR family transcriptional regulator -
U068_RS10445 2069158..2069982 - 825 WP_000754737.1 SDR family oxidoreductase -
U068_RS10450 2070065..2070319 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
U068_RS10455 2070316..2070567 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
U068_RS23740 2070850..2070900 + 51 WP_001364200.1 his operon leader peptide -
U068_RS10460 2071046..2071945 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
U068_RS10465 2071951..2073255 + 1305 WP_000009594.1 histidinol dehydrogenase -
U068_RS10470 2073252..2074322 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
U068_RS10475 2074322..2075389 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T54070 WP_000767829.1 NZ_CP011342:c2070319-2070065 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T54070 NZ_CP011342:c2070319-2070065 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT54070 WP_001259255.1 NZ_CP011342:c2070567-2070316 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT54070 NZ_CP011342:c2070567-2070316 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References