54070

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2070065..2070567 Replicon chromosome
Accession NZ_CP011342
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag U068_RS10450 Protein ID WP_000767829.1
Coordinates 2070065..2070319 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag U068_RS10455 Protein ID WP_001259255.1
Coordinates 2070316..2070567 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U068_RS10425 2065080..2065307 - 228 WP_000234896.1 sulfurtransferase TusA -
U068_RS10430 2065321..2066379 - 1059 WP_000492339.1 transport protein YeeE -
U068_RS10435 2066558..2067916 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
U068_RS25400 2067906..2067968 - 63 WP_010723108.1 membrane protein YoeI -
U068_RS10440 2068183..2069112 - 930 WP_000803351.1 LysR family transcriptional regulator -
U068_RS10445 2069158..2069982 - 825 WP_000754737.1 SDR family oxidoreductase -
U068_RS10450 2070065..2070319 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
U068_RS10455 2070316..2070567 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
U068_RS23740 2070850..2070900 + 51 WP_001364200.1 his operon leader peptide -
U068_RS10460 2071046..2071945 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
U068_RS10465 2071951..2073255 + 1305 WP_000009594.1 histidinol dehydrogenase -
U068_RS10470 2073252..2074322 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
U068_RS10475 2074322..2075389 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T54070 WP_000767829.1 NZ_CP011342:c2070319-2070065 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T54070 NZ_CP011342:c2070319-2070065 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT54070 WP_001259255.1 NZ_CP011342:c2070567-2070316 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT54070 NZ_CP011342:c2070567-2070316 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References