Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 9381..9645 Replicon plasmid unnamed
Accession NZ_CP011333
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag AAF13_RS27225 Protein ID WP_001387489.1
Coordinates 9493..9645 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 9381..9443 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS27210 5483..6553 - 1071 WP_000151588.1 IncI1-type conjugal transfer protein TrbB -
AAF13_RS27215 6572..7780 - 1209 WP_000121263.1 IncI1-type conjugal transfer protein TrbA -
AAF13_RS27220 8087..9178 - 1092 WP_000426061.1 hypothetical protein -
- 9381..9443 - 63 NuclAT_0 - Antitoxin
- 9381..9443 - 63 NuclAT_0 - Antitoxin
- 9381..9443 - 63 NuclAT_0 - Antitoxin
- 9381..9443 - 63 NuclAT_0 - Antitoxin
AAF13_RS27225 9493..9645 + 153 WP_001387489.1 Hok/Gef family protein Toxin
AAF13_RS27230 9717..9968 - 252 WP_001291964.1 hypothetical protein -
AAF13_RS27235 10268..10564 + 297 WP_001275298.1 hypothetical protein -
AAF13_RS32430 10629..10805 - 177 WP_001054898.1 hypothetical protein -
AAF13_RS27250 10988..11197 - 210 WP_001140543.1 hemolysin expression modulator Hha -
AAF13_RS27255 11295..11909 - 615 WP_000578648.1 plasmid IncI1-type surface exclusion protein ExcA -
AAF13_RS27260 11985..14153 - 2169 WP_000698360.1 IncI1-type conjugal transfer membrane protein TraY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..16948 16948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T54032 WP_001387489.1 NZ_CP011333:9493-9645 [Escherichia coli O104:H4 str. C227-11]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T54032 NZ_CP011333:9493-9645 [Escherichia coli O104:H4 str. C227-11]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT54032 NZ_CP011333:c9443-9381 [Escherichia coli O104:H4 str. C227-11]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z417

Antitoxin

Download structure file

References