Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1272877..1273379 Replicon chromosome
Accession NZ_CP011331
Organism Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AAF13_RS06185 Protein ID WP_000767829.1
Coordinates 1273125..1273379 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AAF13_RS06180 Protein ID WP_001259255.1
Coordinates 1272877..1273128 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAF13_RS06160 1268054..1269121 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AAF13_RS06165 1269121..1270191 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
AAF13_RS06170 1270188..1271492 - 1305 WP_001386882.1 histidinol dehydrogenase -
AAF13_RS06175 1271498..1272397 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AAF13_RS28225 1272543..1272593 - 51 WP_001364200.1 his operon leader peptide -
AAF13_RS06180 1272877..1273128 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AAF13_RS06185 1273125..1273379 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AAF13_RS06190 1273462..1274286 + 825 WP_000754758.1 SDR family oxidoreductase -
AAF13_RS06195 1274332..1275261 + 930 WP_000803366.1 LysR family transcriptional regulator -
AAF13_RS31135 1275476..1275538 + 63 WP_010723108.1 membrane protein YoeI -
AAF13_RS06200 1275528..1276886 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AAF13_RS06205 1277065..1278123 + 1059 WP_000492324.1 transport protein YeeE -
AAF13_RS06210 1278137..1278364 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T54010 WP_000767829.1 NZ_CP011331:1273125-1273379 [Escherichia coli O104:H4 str. C227-11]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T54010 NZ_CP011331:1273125-1273379 [Escherichia coli O104:H4 str. C227-11]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT54010 WP_001259255.1 NZ_CP011331:1272877-1273128 [Escherichia coli O104:H4 str. C227-11]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT54010 NZ_CP011331:1272877-1273128 [Escherichia coli O104:H4 str. C227-11]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References