Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2086460..2086962 Replicon chromosome
Accession NZ_CP011320
Organism Escherichia coli strain SQ37

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SG48_RS10525 Protein ID WP_000767829.1
Coordinates 2086460..2086714 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SG48_RS10530 Protein ID WP_001259255.1
Coordinates 2086711..2086962 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SG48_RS10500 2081475..2081702 - 228 WP_000234896.1 sulfurtransferase TusA -
SG48_RS10505 2081716..2082774 - 1059 WP_000492339.1 transport protein YeeE -
SG48_RS10510 2082953..2084311 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SG48_RS25615 2084301..2084363 - 63 WP_010723108.1 membrane protein YoeI -
SG48_RS10515 2084578..2085507 - 930 WP_000803351.1 LysR family transcriptional regulator -
SG48_RS10520 2085553..2086377 - 825 WP_000754737.1 SDR family oxidoreductase -
SG48_RS10525 2086460..2086714 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SG48_RS10530 2086711..2086962 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SG48_RS23975 2087245..2087295 + 51 WP_001364200.1 his operon leader peptide -
SG48_RS10535 2087441..2088340 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
SG48_RS10540 2088346..2089650 + 1305 WP_000009594.1 histidinol dehydrogenase -
SG48_RS10545 2089647..2090717 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SG48_RS10550 2090717..2091784 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53854 WP_000767829.1 NZ_CP011320:c2086714-2086460 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53854 NZ_CP011320:c2086714-2086460 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53854 WP_001259255.1 NZ_CP011320:c2086962-2086711 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53854 NZ_CP011320:c2086962-2086711 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References