Detailed information of TA system TA053826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6401323..6401918
• Replicon: chromosome
• Accession: NZ_CP011317
• Organism: Pseudomonas aeruginosa strain Carb01 63

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: YQ19_RS36905
• Protein ID: WP_003117425.1
• Coordinates: 6401640..6401918 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: YQ19_RS30445
• Protein ID: WP_003099268.1
• Coordinates: 6401323..6401628 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YQ19_RS30405	6396546..6396797	-	252	WP_003115979.1	hypothetical protein	-
YQ19_RS30415	6396919..6397353	-	435	WP_023087663.1	hypothetical protein	-
YQ19_RS30425	6397869..6398159	-	291	WP_033896069.1	DUF5447 family protein	-
YQ19_RS30430	6398335..6398607	-	273	WP_004352675.1	hypothetical protein	-
YQ19_RS30435	6399054..6399389	+	336	WP_025297674.1	hypothetical protein	-
YQ19_RS37395	6399442..6400533	+	1092	WP_124135598.1	hypothetical protein	-
YQ19_RS30440	6400594..6400986	-	393	WP_025297673.1	hypothetical protein	-
YQ19_RS30445	6401323..6401628	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
YQ19_RS36905	6401640..6401918	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YQ19_RS30450	6402243..6404471	+	2229	WP_003113525.1	TonB-dependent receptor	-
YQ19_RS30455	6404541..6405188	-	648	WP_003095021.1	carbonate dehydratase	-
YQ19_RS30460	6405250..6406488	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T53826 WP_003117425.1 NZ_CP011317:c6401918-6401640 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T53826 NZ_CP011317:c6401918-6401640 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT53826 WP_003099268.1 NZ_CP011317:c6401628-6401323 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT53826 NZ_CP011317:c6401628-6401323 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5