Detailed information of TA system TA053735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5540578..5541237
• Replicon: chromosome
• Accession: NZ_CP011254
• Organism: Serratia fonticola strain DSM 4576

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F7HHG5
• Locus tag: WN53_RS24660
• Protein ID: WP_024486065.1
• Coordinates: 5540884..5541237 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WN53_RS24655
• Protein ID: WP_024486066.1
• Coordinates: 5540578..5540880 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN53_RS24635	5535905..5537074	+	1170	WP_046808358.1	flavin-dependent monooxygenase	-
WN53_RS24640	5537095..5538039	+	945	WP_024486069.1	flavin reductase family protein	-
WN53_RS24645	5538112..5539314	-	1203	WP_024486068.1	Bcr/CflA family multidrug efflux MFS transporter	-
WN53_RS24650	5539574..5540434	-	861	WP_024486067.1	aldo/keto reductase family oxidoreductase	-
WN53_RS24655	5540578..5540880	-	303	WP_024486066.1	XRE family transcriptional regulator	Antitoxin
WN53_RS24660	5540884..5541237	-	354	WP_024486065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WN53_RS24665	5541495..5542682	-	1188	WP_024486064.1	sugar transporter	-
WN53_RS24670	5543004..5543615	+	612	WP_024485150.1	GntR family transcriptional regulator	-
WN53_RS24675	5543668..5544819	-	1152	WP_046808359.1	lactaldehyde reductase	-
WN53_RS24680	5544929..5545819	-	891	WP_024485151.1	dihydrodipicolinate synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13575.50 Da
• Isoelectric Point: 9.5694

>T53735 WP_024486065.1 NZ_CP011254:c5541237-5540884 [Serratia fonticola]
VWEIKTTDTFDEWFSRLEDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDPRRRGI
LLCAGSKAGNEKRFYEVMIPIADREFTQFLNTAENKE

Nucleotide

• Length: 354 bp

>T53735 NZ_CP011254:c5541237-5540884 [Serratia fonticola]
GTGTGGGAAATTAAAACAACGGATACGTTTGACGAGTGGTTCAGCCGGCTGGAGGACACTGACAGAGCTTCTGTGCTGGC
GTCACTCATTGTACTACGTGAAAAAGGCCCTCAGTTGCCCAGACCTTATGCCGACACGGTAAAGGGATCGCGCTACAGCA
ACATGAAGGAACTACGCGTGCAAAGCCGAGGTATACCGATACGGGCATTCTTTGCCTTTGATCCACGCCGTCGCGGTATT
TTACTTTGTGCTGGTAGCAAGGCCGGTAATGAGAAGCGTTTTTACGAGGTGATGATCCCGATTGCCGATCGGGAGTTTAC
CCAATTTCTGAATACAGCAGAGAACAAGGAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11017.76 Da
• Isoelectric Point: 6.2326

>AT53735 WP_024486066.1 NZ_CP011254:c5540880-5540578 [Serratia fonticola]
MGRTLEQILAEEKPEVVASAEHIAAETLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT53735 NZ_CP011254:c5540880-5540578 [Serratia fonticola]
ATGGGCAGAACACTGGAACAAATCCTGGCGGAAGAAAAACCCGAGGTAGTGGCCAGCGCTGAGCACATTGCGGCGGAAAC
GCTGCTCAATATTCACTTGGCCGAGCTACGTGAGCGGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCACTTGGCATCA
AACAACCCACCGTCGCGGGTATGGAAAAGCCTGGCCGCGATCTGAAGCTCTCCTCGCTCAAGCGCTATATCGAGGCTGCG
GGGGGCAAGCTGCGTCTGGATATCGAGCTGCCAGATGGTTCGCACTACGGGTTTGTTATTTAA

Structures

Toxin

• AlphaFold DB: A0A0F7HHG5