Detailed information of TA system TA053719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1843984..1844546
• Replicon: chromosome
• Accession: NZ_CP011254
• Organism: Serratia fonticola strain DSM 4576

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3G2FJW6
• Locus tag: WN53_RS08155
• Protein ID: WP_024483282.1
• Coordinates: 1843984..1844262 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WN53_RS08160
• Protein ID: WP_024483281.1
• Coordinates: 1844262..1844546 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN53_RS08140	1839812..1840720	+	909	WP_024483285.1	glycine--tRNA ligase subunit alpha	-
WN53_RS08145	1840729..1842798	+	2070	WP_024483284.1	glycine--tRNA ligase subunit beta	-
WN53_RS08150	1842996..1843715	+	720	WP_024483283.1	DUF3053 domain-containing protein	-
WN53_RS08155	1843984..1844262	+	279	WP_024483282.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WN53_RS08160	1844262..1844546	+	285	WP_024483281.1	HigA family addiction module antidote protein	Antitoxin
WN53_RS08165	1845113..1847029	+	1917	WP_024483280.1	PTS mannitol transporter subunit IICBA	-
WN53_RS08170	1847092..1848240	+	1149	WP_024483279.1	mannitol-1-phosphate 5-dehydrogenase	-
WN53_RS08175	1848390..1848962	+	573	WP_161629419.1	MltR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10486.86 Da
• Isoelectric Point: 9.4604

>T53719 WP_024483282.1 NZ_CP011254:1843984-1844262 [Serratia fonticola]
MIKSFKHKGLRQFFEKGSTAGINTQQIGKIRLRLGVIDAAKDISDINRPGYQLHPLSGDRAGQWSITVTGNWRITFEFID
GDAYIVNYEDYH

Nucleotide

• Length: 279 bp

>T53719 NZ_CP011254:1843984-1844262 [Serratia fonticola]
ATGATTAAGAGTTTTAAGCACAAGGGATTGCGACAGTTTTTTGAGAAAGGCTCTACGGCTGGCATCAATACTCAACAGAT
CGGTAAAATTCGTCTGAGGCTTGGTGTAATTGATGCAGCAAAGGACATCAGTGATATCAACCGCCCTGGCTATCAACTTC
ACCCATTGTCGGGTGACCGTGCCGGGCAGTGGTCAATAACCGTAACAGGCAACTGGCGCATCACGTTTGAATTTATCGAC
GGTGATGCCTATATCGTCAACTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10107.83 Da
• Isoelectric Point: 8.7051

>AT53719 WP_024483281.1 NZ_CP011254:1844262-1844546 [Serratia fonticola]
MAMHNPPHPGEIIAETLEELGVGIRVFARALMVAPSTAQRLVAGNAAVSPEMAVKLSIVLGSSPEFWLNLQDNYSLWIAK
KAVNTSQLKRLVAA

Nucleotide

• Length: 285 bp

>AT53719 NZ_CP011254:1844262-1844546 [Serratia fonticola]
ATGGCTATGCATAACCCCCCGCATCCGGGCGAGATCATCGCTGAAACGCTGGAAGAACTGGGTGTTGGTATCCGCGTATT
TGCCAGGGCGTTGATGGTGGCACCCTCCACCGCACAGCGCTTGGTCGCAGGGAATGCGGCTGTTAGCCCTGAGATGGCGG
TGAAGTTGTCCATAGTGCTGGGCAGTTCCCCTGAATTCTGGCTAAACCTGCAGGATAATTACAGCCTGTGGATCGCCAAA
AAAGCAGTCAATACTTCGCAGCTAAAGCGTTTGGTTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A3G2FJW6