Detailed information of TA system TA053554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80292..80546
• Replicon: plasmid pVR50B
• Accession: NZ_CP011136
• Organism: Escherichia coli VR50

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: ECVR50_RS25960
• Protein ID: WP_001362723.1
• Coordinates: 80292..80498 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	80485..80546 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECVR50_RS25920	75857..76096	+	240	WP_001554944.1	hypothetical protein	-
ECVR50_RS30405	76246..76344	-	99	WP_172400466.1	transposase	-
ECVR50_RS25930	76537..76845	+	309	Protein_79	transposase	-
ECVR50_RS29675	77765..78109	-	345	Protein_80	incFII family plasmid replication initiator RepA	-
ECVR50_RS25945	78098..78802	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ECVR50_RS25950	78854..79449	-	596	Protein_82	incFII family plasmid replication initiator RepA	-
ECVR50_RS28425	79442..79516	-	75	WP_057113837.1	RepA leader peptide Tap	-
ECVR50_RS25955	79751..80008	-	258	WP_000083845.1	replication regulatory protein RepA	-
ECVR50_RS25960	80292..80498	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	80485..80546	+	62	NuclAT_0	-	Antitoxin
-	80485..80546	+	62	NuclAT_0	-	Antitoxin
-	80485..80546	+	62	NuclAT_0	-	Antitoxin
-	80485..80546	+	62	NuclAT_0	-	Antitoxin
ECVR50_RS25965	80841..81152	+	312	WP_000802277.1	hypothetical protein	-
ECVR50_RS30410	81333..81419	-	87	Protein_87	endonuclease	-
ECVR50_RS25970	81664..81876	-	213	WP_001301409.1	ANR family transcriptional regulator	-
ECVR50_RS25975	82008..82568	-	561	WP_000139325.1	fertility inhibition protein FinO	-
ECVR50_RS25980	82623..83369	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	senB	1..97864	97864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T53554 WP_001362723.1 NZ_CP011136:c80498-80292 [Escherichia coli VR50]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T53554 NZ_CP011136:c80498-80292 [Escherichia coli VR50]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT53554 NZ_CP011136:80485-80546 [Escherichia coli VR50]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin