Detailed information of TA system TA053546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3479429..3480122
• Replicon: chromosome
• Accession: NZ_CP011134
• Organism: Escherichia coli VR50

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: U9ZN09
• Locus tag: ECVR50_RS17415
• Protein ID: WP_000415585.1
• Coordinates: 3479826..3480122 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECVR50_RS17410
• Protein ID: WP_000650107.1
• Coordinates: 3479429..3479824 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECVR50_RS17400	3475293..3477551	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
ECVR50_RS17405	3477689..3479296	-	1608	WP_046072893.1	ABC transporter substrate-binding protein	-
ECVR50_RS17410	3479429..3479824	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECVR50_RS17415	3479826..3480122	-	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECVR50_RS17420	3480327..3480809	-	483	WP_000183505.1	transcriptional regulator	-
ECVR50_RS17425	3480862..3481254	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECVR50_RS17430	3481406..3482065	+	660	WP_001221493.1	two-component system response regulator QseB	-
ECVR50_RS17435	3482062..3483411	+	1350	WP_000673357.1	two-component system sensor histidine kinase QseC	-
ECVR50_RS17440	3483457..3483789	-	333	WP_000914691.1	DUF2645 family protein	-
ECVR50_RS29460	3483796..3484988	-	1193	Protein_3294	Hcp family type VI secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	draP / afaD / afaC-I / afaB-I / afaA / papX / papA / papI / sat / iutA / iucD / iucC / iucB / iucA / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	3297487..3499887	202400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11261.99 Da
• Isoelectric Point: 8.9070

>T53546 WP_000415585.1 NZ_CP011134:c3480122-3479826 [Escherichia coli VR50]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T53546 NZ_CP011134:c3480122-3479826 [Escherichia coli VR50]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT53546 WP_000650107.1 NZ_CP011134:c3479824-3479429 [Escherichia coli VR50]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT53546 NZ_CP011134:c3479824-3479429 [Escherichia coli VR50]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: U9ZN09

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65