Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2203928..2204430 Replicon chromosome
Accession NZ_CP011134
Organism Escherichia coli VR50

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECVR50_RS11090 Protein ID WP_000767829.1
Coordinates 2203928..2204182 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECVR50_RS11095 Protein ID WP_001259255.1
Coordinates 2204179..2204430 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS11065 2198943..2199170 - 228 WP_000234896.1 sulfurtransferase TusA -
ECVR50_RS11070 2199184..2200242 - 1059 WP_000492339.1 transport protein YeeE -
ECVR50_RS11075 2200421..2201779 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECVR50_RS29325 2201769..2201831 - 63 WP_010723108.1 membrane protein YoeI -
ECVR50_RS11080 2202046..2202975 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECVR50_RS11085 2203021..2203845 - 825 WP_000754737.1 SDR family oxidoreductase -
ECVR50_RS11090 2203928..2204182 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECVR50_RS11095 2204179..2204430 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECVR50_RS27175 2204713..2204763 + 51 WP_001364200.1 his operon leader peptide -
ECVR50_RS11100 2204909..2205808 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECVR50_RS11105 2205814..2207118 + 1305 WP_000009594.1 histidinol dehydrogenase -
ECVR50_RS11110 2207115..2208185 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ECVR50_RS11115 2208185..2209252 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53542 WP_000767829.1 NZ_CP011134:c2204182-2203928 [Escherichia coli VR50]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53542 NZ_CP011134:c2204182-2203928 [Escherichia coli VR50]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53542 WP_001259255.1 NZ_CP011134:c2204430-2204179 [Escherichia coli VR50]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53542 NZ_CP011134:c2204430-2204179 [Escherichia coli VR50]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References