Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 290330..291024 Replicon chromosome
Accession NZ_CP011134
Organism Escherichia coli VR50

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag ECVR50_RS01350 Protein ID WP_001263488.1
Coordinates 290626..291024 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ECVR50_RS01345 Protein ID WP_000554758.1
Coordinates 290330..290623 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS01325 285962..286459 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECVR50_RS01330 286683..288395 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
ECVR50_RS01335 288367..289152 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECVR50_RS01340 289223..290278 + 1056 WP_001226164.1 DNA polymerase IV -
ECVR50_RS01345 290330..290623 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ECVR50_RS01350 290626..291024 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ECVR50_RS01355 291034..291486 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
ECVR50_RS01360 291804..292010 + 207 Protein_258 RtcB family protein -
ECVR50_RS01365 292006..292527 + 522 Protein_259 peptide chain release factor H -
ECVR50_RS01370 292584..294041 - 1458 WP_046072789.1 cytosol nonspecific dipeptidase -
ECVR50_RS01375 294302..294760 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 295356..295436 + 81 NuclAT_10 - -
- 295356..295436 + 81 NuclAT_10 - -
- 295356..295436 + 81 NuclAT_10 - -
- 295356..295436 + 81 NuclAT_10 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T53530 WP_001263488.1 NZ_CP011134:290626-291024 [Escherichia coli VR50]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T53530 NZ_CP011134:290626-291024 [Escherichia coli VR50]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT53530 WP_000554758.1 NZ_CP011134:290330-290623 [Escherichia coli VR50]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT53530 NZ_CP011134:290330-290623 [Escherichia coli VR50]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References