Detailed information of TA system TA053524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4562580..4563182
• Replicon: chromosome
• Accession: NZ_CP011132
• Organism: Citrobacter amalonaticus Y19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6TYA5
• Locus tag: F384_RS21045
• Protein ID: WP_046493129.1
• Coordinates: 4562580..4562891 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F384_RS21050
• Protein ID: WP_046493132.1
• Coordinates: 4562892..4563182 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F384_RS21020	4558513..4559112	+	600	WP_046493119.1	glucose-1-phosphatase	-
F384_RS21025	4559106..4559978	+	873	WP_046493122.1	virulence factor BrkB family protein	-
F384_RS21030	4559975..4560412	+	438	WP_046493123.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F384_RS21035	4560457..4561398	+	942	WP_046493125.1	fatty acid biosynthesis protein FabY	-
F384_RS21040	4561414..4562346	-	933	WP_046493127.1	alpha/beta hydrolase	-
F384_RS21045	4562580..4562891	+	312	WP_046493129.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F384_RS21050	4562892..4563182	+	291	WP_046493132.1	helix-turn-helix domain-containing protein	Antitoxin
F384_RS21055	4563234..4564163	-	930	WP_046493134.1	formate dehydrogenase accessory protein FdhE	-
F384_RS21060	4564308..4564994	-	687	WP_046493136.1	response regulator transcription factor	-
F384_RS21065	4564999..4566495	-	1497	WP_046493139.1	HAMP domain-containing histidine kinase	-
F384_RS21070	4566513..4567850	-	1338	WP_046493142.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12401.24 Da
• Isoelectric Point: 9.3562

>T53524 WP_046493129.1 NZ_CP011132:4562580-4562891 [Citrobacter amalonaticus Y19]
MIFIETEFFTEDVQQLLDDDEYHRFQLFLAAQPEYGDVIQDTGGLRKIRWKAQGKGKRGGVRIIYFYQSRRTEVRLLLIY
RKGLKDDLTQQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T53524 NZ_CP011132:4562580-4562891 [Citrobacter amalonaticus Y19]
ATGATATTCATAGAAACGGAATTTTTTACTGAAGATGTCCAGCAACTGTTGGACGACGATGAATACCATCGATTTCAGCT
GTTCCTGGCTGCGCAGCCCGAGTATGGCGATGTGATTCAAGATACTGGCGGCTTGAGAAAAATTCGTTGGAAAGCTCAGG
GGAAGGGAAAGCGAGGTGGTGTCCGGATCATCTACTTTTATCAGAGCAGGCGTACAGAAGTTCGGTTATTGCTGATTTAT
CGAAAAGGTCTCAAGGATGACCTGACCCAGCAAGAAAAAGCAGTGCTCCGTATGTTAAATGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10737.47 Da
• Isoelectric Point: 10.7588

>AT53524 WP_046493132.1 NZ_CP011132:4562892-4563182 [Citrobacter amalonaticus Y19]
MDKALFERLTKSMNQMNEIVEGARLPSRIFHIDAMKIKEIRQASGLSQSKFADLISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPLHVIPALAQ

Nucleotide

• Length: 291 bp

>AT53524 NZ_CP011132:4562892-4563182 [Citrobacter amalonaticus Y19]
ATGGATAAGGCGCTATTCGAGCGACTGACTAAAAGCATGAACCAAATGAACGAGATAGTTGAAGGTGCACGTCTACCTTC
ACGAATTTTTCATATTGATGCGATGAAAATTAAAGAAATCCGTCAGGCTTCGGGGCTGTCGCAATCGAAGTTTGCCGATC
TGATATCAGTCAATGTTGATACCTTACGCAATTGGGAACAGGGCAGACGTTCCCCAACGGGGCCAGCAAAAGCATTGCTA
CGGGCAATTGCTAACGATCCGCTGCATGTCATCCCTGCGCTGGCGCAATAA

Structures

Toxin

• AlphaFold DB: A0A0F6TYA5