Detailed information of TA system TA053513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1591598..1592160
• Replicon: chromosome
• Accession: NZ_CP011132
• Organism: Citrobacter amalonaticus Y19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M1K6R8
• Locus tag: F384_RS07165
• Protein ID: WP_046497865.1
• Coordinates: 1591598..1591876 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F384_RS07170
• Protein ID: WP_043000693.1
• Coordinates: 1591876..1592160 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F384_RS07145	1586723..1587154	-	432	WP_046480856.1	peroxiredoxin OsmC	-
F384_RS07150	1587381..1587524	+	144	WP_046480858.1	stationary-phase-induced ribosome-associated protein	-
F384_RS07155	1587697..1589394	+	1698	WP_046480860.1	malate dehydrogenase	-
F384_RS07160	1589703..1591418	+	1716	WP_046480861.1	ATP-binding cassette domain-containing protein	-
F384_RS07165	1591598..1591876	+	279	WP_046497865.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F384_RS07170	1591876..1592160	+	285	WP_043000693.1	HigA family addiction module antidote protein	Antitoxin
F384_RS07175	1592211..1592813	-	603	WP_046480863.1	inorganic diphosphatase	-
F384_RS07180	1592957..1593613	-	657	WP_046480865.1	formate dehydrogenase-N subunit gamma	-
F384_RS07185	1593606..1594490	-	885	WP_046480867.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10635.16 Da
• Isoelectric Point: 8.1156

>T53513 WP_046497865.1 NZ_CP011132:1591598-1591876 [Citrobacter amalonaticus Y19]
MIMNFRHKGLRDLFLHGKTSGVMTNHVRRLRHRLAVIDAAGCVNDINMPGYRLHLLSGDRDGVWAISVSGNWRMTFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T53513 NZ_CP011132:1591598-1591876 [Citrobacter amalonaticus Y19]
ATGATCATGAATTTCAGGCACAAGGGATTACGCGACCTGTTTCTTCACGGCAAAACGTCTGGTGTGATGACAAATCACGT
CAGACGTTTACGACACCGCCTTGCTGTGATTGATGCCGCAGGCTGCGTGAACGATATCAACATGCCCGGTTACCGGCTGC
ATCTCCTGAGCGGCGATCGCGATGGCGTCTGGGCGATTTCGGTTTCAGGCAACTGGCGTATGACGTTTGAATTCGTCAAT
GGCGATGCGTACATTCTGGATTACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT53513 WP_043000693.1 NZ_CP011132:1591876-1592160 [Citrobacter amalonaticus Y19]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT53513 NZ_CP011132:1591876-1592160 [Citrobacter amalonaticus Y19]
ATGAAAATGGCGAATCATCCCCGCCCCGGGGATATCATTCAGGAAGCGCTGGACGAACTGAACGTCAGTTTGCGTGAATT
TGCCCGAGCAATGGAGATTGCGCCCTCAACGGCAAGTCGGTTACTCACGGGTAAAGCGGCCCTGACCCCGGAAATGGCGA
TCAAACTCTCGGTCGTGATCGGCAGCTCTCCCGAAATGTGGCTGAATCTGCAAAATACCTGGAGTCTGGCTCAGGCGCAA
AAAAGCGTGGACGTTTCCCGCCTGCGCCGACTGGCCGTACAGTAA

Structures

Toxin

• AlphaFold DB: M1K6R8