53510

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 554998..555648 Replicon chromosome
Accession NZ_CP011132
Organism Citrobacter amalonaticus Y19

Toxin (Protein)


Gene name higB Uniprot ID A0A0F6TTJ4
Locus tag F384_RS02420 Protein ID WP_046477155.1
Coordinates 555307..555648 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0F6RE90
Locus tag F384_RS02415 Protein ID WP_046477154.1
Coordinates 554998..555297 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F384_RS02400 550122..551207 + 1086 WP_046477149.1 YncE family protein -
F384_RS02405 551272..553455 + 2184 WP_046477151.1 TonB-dependent siderophore receptor -
F384_RS02410 553572..554960 + 1389 WP_046477152.1 phenylalanine transporter -
F384_RS02415 554998..555297 - 300 WP_046477154.1 helix-turn-helix transcriptional regulator Antitoxin
F384_RS02420 555307..555648 - 342 WP_046477155.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F384_RS02425 555858..557096 + 1239 WP_046477157.1 MFS transporter -
F384_RS02430 557112..558137 + 1026 WP_046477158.1 sugar phosphate isomerase/epimerase -
F384_RS02435 558130..559272 + 1143 WP_046477160.1 Gfo/Idh/MocA family oxidoreductase -
F384_RS02440 559348..560319 + 972 WP_046477162.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-87)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13116.12 Da        Isoelectric Point: 6.4615

>T53510 WP_046477155.1 NZ_CP011132:c555648-555307 [Citrobacter amalonaticus Y19]
MWDVETTERFDEWFYQQTLALKEDVLAAMYILAEYGPQLGRPYVDTVKASTYPNMKELRIQHAGTPIRAFFAFDPSRKAI
VLCAGDKTGRLEKQFYKEMIKLADAEFCQHLAK

Download         Length: 342 bp

>T53510 NZ_CP011132:c555648-555307 [Citrobacter amalonaticus Y19]
ATGTGGGACGTCGAAACAACAGAACGGTTTGATGAATGGTTCTATCAACAAACCTTGGCATTAAAAGAGGATGTGCTGGC
TGCAATGTATATTCTTGCAGAGTATGGCCCACAACTTGGACGTCCCTACGTTGATACAGTGAAAGCGTCAACCTATCCAA
ATATGAAGGAATTACGCATTCAGCATGCGGGAACACCAATCAGAGCATTCTTTGCTTTTGATCCCAGCCGTAAAGCGATC
GTGTTGTGCGCCGGGGATAAAACGGGACGCCTGGAAAAACAATTCTACAAAGAGATGATTAAGCTGGCAGATGCTGAGTT
CTGCCAGCATCTGGCGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10956.70 Da        Isoelectric Point: 7.0154

>AT53510 WP_046477154.1 NZ_CP011132:c555297-554998 [Citrobacter amalonaticus Y19]
MATLKELMAQQSVESQERIATKVEEMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEHPGNDPRLSTLKRYVAALG
GELSIDVTLPDGKRIAFQI

Download         Length: 300 bp

>AT53510 NZ_CP011132:c555297-554998 [Citrobacter amalonaticus Y19]
ATGGCTACGCTGAAAGAACTGATGGCGCAACAGAGCGTGGAAAGTCAGGAGCGCATTGCTACTAAAGTTGAAGAGATGCG
CCGTATCGTTGCACTCAACCAGTTGCGCGAAGAGTTGAACATCTCGCAAACCGAACTCGCTGCGGCAATGGGAGTAAAAC
AGCCCACGGTGGCTAAAATTGAACATCCCGGTAACGACCCTCGACTGTCTACTCTGAAACGCTACGTAGCGGCGCTTGGG
GGAGAACTGAGTATTGATGTGACCTTACCTGATGGGAAACGGATAGCTTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6TTJ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RE90

References