Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4481447..4481988 Replicon chromosome
Accession NZ_CP011124
Organism Escherichia coli strain USML2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag USML2_RS22945 Protein ID WP_000615983.1
Coordinates 4481710..4481988 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag USML2_RS22940 Protein ID WP_000729703.1
Coordinates 4481447..4481707 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USML2_RS22920 4477392..4478405 - 1014 WP_001013499.1 4-hydroxy-2-oxovalerate aldolase -
USML2_RS22925 4478395..4479591 + 1197 Protein_4186 flagellar biosynthesis protein FlhA -
USML2_RS22930 4479815..4480312 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
USML2_RS22935 4480488..4481237 - 750 WP_000056849.1 C40 family peptidase -
USML2_RS22940 4481447..4481707 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
USML2_RS22945 4481710..4481988 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
USML2_RS22950 4482144..4482884 + 741 WP_001225679.1 murein L,D-transpeptidase -
USML2_RS22955 4482855..4483622 - 768 WP_000333380.1 class II glutamine amidotransferase -
USML2_RS22960 4483828..4484406 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island formA gmhA/lpcA 4467657..4493922 26265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T53507 WP_000615983.1 NZ_CP011124:4481710-4481988 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T53507 NZ_CP011124:4481710-4481988 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT53507 WP_000729703.1 NZ_CP011124:4481447-4481707 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT53507 NZ_CP011124:4481447-4481707 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References