Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 1635106..1635721 | Replicon | chromosome |
| Accession | NC_003364 | ||
| Organism | Pyrobaculum aerophilum str. IM2 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | PAE_RS09225 | Protein ID | WP_011008882.1 |
| Coordinates | 1635106..1635507 (-) | Length | 134 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | PAE_RS09230 | Protein ID | WP_011008883.1 |
| Coordinates | 1635497..1635721 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PAE_RS09195 (PAE2746) | 1631049..1631228 | - | 180 | WP_011008876.1 | YHS domain-containing protein | - |
| PAE_RS09200 (PAE2747) | 1631265..1631861 | - | 597 | WP_011008877.1 | hypothetical protein | - |
| PAE_RS09205 (PAE2748) | 1631965..1632441 | + | 477 | WP_011008878.1 | hypothetical protein | - |
| PAE_RS09210 (PAE2750) | 1632711..1633220 | + | 510 | WP_011008879.1 | DUF411 domain-containing protein | - |
| PAE_RS09215 (PAE2751) | 1633307..1634227 | + | 921 | WP_226976122.1 | thioredoxin family protein | - |
| PAE_RS09220 (PAE2753) | 1634659..1634985 | + | 327 | WP_011008881.1 | hypothetical protein | - |
| PAE_RS09225 (PAE2754) | 1635106..1635507 | - | 402 | WP_011008882.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| PAE_RS09230 (PAE2755) | 1635497..1635721 | - | 225 | WP_011008883.1 | type II toxin-antitoxin system CcdA family antitoxin | Antitoxin |
| PAE_RS13885 (PAE2756) | 1635906..1636031 | + | 126 | WP_264357544.1 | hypothetical protein | - |
| PAE_RS09235 (PAE2758) | 1636096..1638465 | + | 2370 | WP_011008885.1 | heavy metal translocating P-type ATPase | - |
| PAE_RS09240 (PAE2759) | 1638507..1638869 | + | 363 | WP_011008886.1 | DUF86 domain-containing protein | - |
| PAE_RS09245 (PAE2760) | 1638885..1639247 | + | 363 | WP_011008887.1 | nucleotidyltransferase domain-containing protein | - |
| PAE_RS09250 (PAE2762) | 1639309..1639815 | - | 507 | WP_011008888.1 | DUF996 domain-containing protein | - |
| PAE_RS13115 (PAE2766) | 1640019..1640171 | + | 153 | WP_011008889.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 134 a.a. Molecular weight: 14909.26 Da Isoelectric Point: 6.8815
>T535 WP_011008882.1 NC_003364:c1635507-1635106 [Pyrobaculum aerophilum str. IM2]
MPVEYLVDASALYALAAHYDKWIKHREKLAILHLTIYEAGNALWKEARLGRVDWAAASRHLKKVLSSFKVLEDPPLDEVL
RVAVERGLTFYDASYAYVAESSGLVLVTQDRELLAKTKGAIDVETLLVRLAAQ
MPVEYLVDASALYALAAHYDKWIKHREKLAILHLTIYEAGNALWKEARLGRVDWAAASRHLKKVLSSFKVLEDPPLDEVL
RVAVERGLTFYDASYAYVAESSGLVLVTQDRELLAKTKGAIDVETLLVRLAAQ
Download Length: 402 bp
>T535 NC_003364:c1635507-1635106 [Pyrobaculum aerophilum str. IM2]
ATGCCCGTTGAGTACCTAGTGGACGCCTCCGCGCTATACGCCCTCGCGGCCCATTACGACAAGTGGATCAAACATAGGGA
GAAACTGGCCATTCTGCACTTGACCATATACGAGGCAGGCAACGCGTTGTGGAAAGAGGCGAGGCTCGGGAGAGTGGACT
GGGCCGCCGCGTCTCGGCATTTGAAAAAGGTGTTGTCCAGCTTCAAGGTGTTGGAGGACCCGCCCCTAGACGAGGTCTTG
AGGGTGGCCGTGGAGCGGGGCTTGACCTTCTACGACGCCAGCTACGCCTACGTGGCGGAGTCCTCCGGACTAGTCTTGGT
GACGCAAGACCGCGAGCTACTGGCCAAGACGAAAGGCGCTATAGACGTCGAAACTTTACTGGTAAGGCTGGCGGCACAAT
AA
ATGCCCGTTGAGTACCTAGTGGACGCCTCCGCGCTATACGCCCTCGCGGCCCATTACGACAAGTGGATCAAACATAGGGA
GAAACTGGCCATTCTGCACTTGACCATATACGAGGCAGGCAACGCGTTGTGGAAAGAGGCGAGGCTCGGGAGAGTGGACT
GGGCCGCCGCGTCTCGGCATTTGAAAAAGGTGTTGTCCAGCTTCAAGGTGTTGGAGGACCCGCCCCTAGACGAGGTCTTG
AGGGTGGCCGTGGAGCGGGGCTTGACCTTCTACGACGCCAGCTACGCCTACGTGGCGGAGTCCTCCGGACTAGTCTTGGT
GACGCAAGACCGCGAGCTACTGGCCAAGACGAAAGGCGCTATAGACGTCGAAACTTTACTGGTAAGGCTGGCGGCACAAT
AA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8794.13 Da Isoelectric Point: 10.5614
>AT535 WP_011008883.1 NC_003364:c1635721-1635497 [Pyrobaculum aerophilum str. IM2]
VSVIISVRVRRELKEKAKRLGIDIRRVVERALEEEIKRREEEELAKSLEELRRALSGISEREWVEAVREARNAR
VSVIISVRVRRELKEKAKRLGIDIRRVVERALEEEIKRREEEELAKSLEELRRALSGISEREWVEAVREARNAR
Download Length: 225 bp
>AT535 NC_003364:c1635721-1635497 [Pyrobaculum aerophilum str. IM2]
GTGTCAGTCATCATCAGCGTAAGAGTGAGGAGGGAGTTGAAGGAGAAGGCCAAACGGCTGGGTATAGATATCAGGAGGGT
GGTCGAGAGGGCGCTGGAGGAAGAGATCAAAAGGCGCGAGGAAGAGGAGCTCGCGAAATCGCTGGAGGAGCTGAGGAGGG
CGCTGTCGGGCATCTCGGAGAGGGAGTGGGTGGAAGCGGTGAGGGAGGCGCGAAATGCCCGTTGA
GTGTCAGTCATCATCAGCGTAAGAGTGAGGAGGGAGTTGAAGGAGAAGGCCAAACGGCTGGGTATAGATATCAGGAGGGT
GGTCGAGAGGGCGCTGGAGGAAGAGATCAAAAGGCGCGAGGAAGAGGAGCTCGCGAAATCGCTGGAGGAGCTGAGGAGGG
CGCTGTCGGGCATCTCGGAGAGGGAGTGGGTGGAAGCGGTGAGGGAGGCGCGAAATGCCCGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T516 | Pyrobaculum aerophilum str. IM2 |
36.364 |
92.366 |
0.336 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Joanna L McKenzie et al. (2012) Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry. RNA (New York, N.Y.) 18(6):1267-78. [PubMed:22539524]