Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 81756..82368 | Replicon | chromosome |
| Accession | NC_003364 | ||
| Organism | Pyrobaculum aerophilum str. IM2 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | PAE_RS00470 | Protein ID | WP_011007068.1 |
| Coordinates | 81756..82151 (-) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | PAE_RS00475 | Protein ID | WP_011007069.1 |
| Coordinates | 82135..82368 (-) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PAE_RS00440 (PAE0142) | 77849..78304 | + | 456 | WP_011007062.1 | hypothetical protein | - |
| PAE_RS00445 (PAE0143) | 78314..78655 | + | 342 | WP_011007063.1 | hypothetical protein | - |
| PAE_RS00450 (PAE0144) | 78825..79163 | - | 339 | WP_011007064.1 | gamma-glutamylcyclotransferase | - |
| PAE_RS00455 (PAE0146) | 79166..80065 | - | 900 | WP_011007065.1 | winged helix-turn-helix transcriptional regulator | - |
| PAE_RS00460 (PAE0148) | 80100..81050 | - | 951 | WP_011007066.1 | hypothetical protein | - |
| PAE_RS13350 | 81313..81474 | + | 162 | Protein_97 | PaREP1 family protein | - |
| PAE_RS12970 (PAE0150) | 81485..81628 | + | 144 | WP_011007067.1 | hypothetical protein | - |
| PAE_RS00470 (PAE0151) | 81756..82151 | - | 396 | WP_011007068.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| PAE_RS00475 (PAE0152) | 82135..82368 | - | 234 | WP_011007069.1 | antitoxin | Antitoxin |
| PAE_RS00480 (PAE0153) | 82432..83343 | - | 912 | WP_011007070.1 | ParA family protein | - |
| PAE_RS00485 (PAE0154) | 83334..83693 | - | 360 | WP_011007071.1 | hypothetical protein | - |
| PAE_RS12975 (PAE0157) | 83902..84066 | + | 165 | WP_158296290.1 | hypothetical protein | - |
| PAE_RS00490 (PAE0158) | 84063..84311 | - | 249 | WP_011007073.1 | hypothetical protein | - |
| PAE_RS00500 (PAE0163) | 84923..85396 | + | 474 | WP_011007075.1 | NUDIX domain-containing protein | - |
| PAE_RS00505 (PAE0164) | 85425..86489 | + | 1065 | WP_011007076.1 | 4-demethylwyosine synthase TYW1 | - |
| PAE_RS00510 (PAE0165) | 86727..87071 | + | 345 | WP_011007078.1 | DUF86 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 14624.78 Da Isoelectric Point: 4.9984
>T516 WP_011007068.1 NC_003364:c82151-81756 [Pyrobaculum aerophilum str. IM2]
MKLVVDASAIAALYVPEERSEQAERAVSQAQELHTLDLAAYEVANDLWKHARRGLLREDEASNMLEELWEFFKALKVHSY
AEVLKDAFALALKHGVTVYDAAYVALAEKIGGKLLTLDRQLAEKFPALVTP
MKLVVDASAIAALYVPEERSEQAERAVSQAQELHTLDLAAYEVANDLWKHARRGLLREDEASNMLEELWEFFKALKVHSY
AEVLKDAFALALKHGVTVYDAAYVALAEKIGGKLLTLDRQLAEKFPALVTP
Download Length: 396 bp
>T516 NC_003364:c82151-81756 [Pyrobaculum aerophilum str. IM2]
ATGAAGCTAGTCGTTGACGCCTCGGCCATAGCCGCGTTGTACGTCCCCGAGGAGCGGAGTGAGCAGGCGGAGAGGGCAGT
GAGCCAGGCCCAAGAGCTACACACCCTAGACCTAGCCGCATATGAAGTCGCCAATGACCTGTGGAAACACGCAAGGCGCG
GGTTGCTCCGGGAGGACGAGGCGAGCAACATGCTGGAGGAGCTGTGGGAATTCTTCAAGGCGTTAAAAGTACACTCCTAC
GCTGAGGTTTTAAAAGACGCCTTCGCCCTGGCTTTGAAACACGGCGTCACTGTTTACGACGCCGCGTACGTCGCCCTCGC
GGAGAAAATAGGGGGCAAATTACTCACCCTAGATAGACAATTGGCCGAGAAGTTTCCAGCCCTGGTCACTCCCTGA
ATGAAGCTAGTCGTTGACGCCTCGGCCATAGCCGCGTTGTACGTCCCCGAGGAGCGGAGTGAGCAGGCGGAGAGGGCAGT
GAGCCAGGCCCAAGAGCTACACACCCTAGACCTAGCCGCATATGAAGTCGCCAATGACCTGTGGAAACACGCAAGGCGCG
GGTTGCTCCGGGAGGACGAGGCGAGCAACATGCTGGAGGAGCTGTGGGAATTCTTCAAGGCGTTAAAAGTACACTCCTAC
GCTGAGGTTTTAAAAGACGCCTTCGCCCTGGCTTTGAAACACGGCGTCACTGTTTACGACGCCGCGTACGTCGCCCTCGC
GGAGAAAATAGGGGGCAAATTACTCACCCTAGATAGACAATTGGCCGAGAAGTTTCCAGCCCTGGTCACTCCCTGA
Antitoxin
Download Length: 78 a.a. Molecular weight: 9087.46 Da Isoelectric Point: 10.8895
>AT516 WP_011007069.1 NC_003364:c82368-82135 [Pyrobaculum aerophilum str. IM2]
VSEVISIRVRRGLKKELEELGINYAEAVRKFLEELVARERRRRALERARALREELRKKGAFPPSAELIREDRDEASR
VSEVISIRVRRGLKKELEELGINYAEAVRKFLEELVARERRRRALERARALREELRKKGAFPPSAELIREDRDEASR
Download Length: 234 bp
>AT516 NC_003364:c82368-82135 [Pyrobaculum aerophilum str. IM2]
GTGAGTGAGGTAATTTCCATTAGGGTGAGGAGAGGGCTGAAAAAAGAACTCGAAGAATTAGGCATAAACTACGCAGAGGC
TGTGAGGAAGTTCCTAGAGGAGCTAGTGGCCAGGGAGAGGAGGAGGCGGGCGCTGGAGAGGGCGAGGGCGCTGAGAGAGG
AGTTGAGGAAAAAAGGAGCCTTCCCCCCAAGCGCTGAGCTTATAAGAGAGGACAGAGATGAAGCTAGTCGTTGA
GTGAGTGAGGTAATTTCCATTAGGGTGAGGAGAGGGCTGAAAAAAGAACTCGAAGAATTAGGCATAAACTACGCAGAGGC
TGTGAGGAAGTTCCTAGAGGAGCTAGTGGCCAGGGAGAGGAGGAGGCGGGCGCTGGAGAGGGCGAGGGCGCTGAGAGAGG
AGTTGAGGAAAAAAGGAGCCTTCCCCCCAAGCGCTGAGCTTATAAGAGAGGACAGAGATGAAGCTAGTCGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T535 | Pyrobaculum aerophilum str. IM2 |
36.364 |
92.366 |
0.336 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Joanna L McKenzie et al. (2012) Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry. RNA (New York, N.Y.) 18(6):1267-78. [PubMed:22539524]