Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2785106..2785608 Replicon chromosome
Accession NZ_CP011124
Organism Escherichia coli strain USML2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag USML2_RS13980 Protein ID WP_000767829.1
Coordinates 2785354..2785608 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag USML2_RS13975 Protein ID WP_001259255.1
Coordinates 2785106..2785357 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USML2_RS13945 2780284..2781351 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
USML2_RS13950 2781351..2782421 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
USML2_RS13955 2782418..2783722 - 1305 WP_000009594.1 histidinol dehydrogenase -
USML2_RS13960 2783728..2784627 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
USML2_RS13970 2784773..2784823 - 51 WP_001364200.1 his operon leader peptide -
USML2_RS13975 2785106..2785357 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
USML2_RS13980 2785354..2785608 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
USML2_RS13985 2785691..2786515 + 825 WP_000754737.1 SDR family oxidoreductase -
USML2_RS13990 2786561..2787490 + 930 WP_000803351.1 LysR family transcriptional regulator -
USML2_RS23240 2787705..2787767 + 63 WP_010723108.1 membrane protein YoeI -
USML2_RS13995 2787757..2789115 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
USML2_RS14000 2789294..2790352 + 1059 WP_000492339.1 transport protein YeeE -
USML2_RS14005 2790366..2790593 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53493 WP_000767829.1 NZ_CP011124:2785354-2785608 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53493 NZ_CP011124:2785354-2785608 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53493 WP_001259255.1 NZ_CP011124:2785106-2785357 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53493 NZ_CP011124:2785106-2785357 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References