Detailed information of TA system TA053456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 508371..508954
• Replicon: chromosome
• Accession: NZ_CP011118
• Organism: Yersinia enterocolitica strain FORC_002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: FORC2_RS02220
• Protein ID: WP_005162526.1
• Coordinates: 508676..508954 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC2_RS02215
• Protein ID: WP_005162529.1
• Coordinates: 508371..508676 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC2_RS02200	504225..504965	+	741	WP_005174290.1	KDGP aldolase family protein	-
FORC2_RS02205	505075..506298	+	1224	WP_046694479.1	lactonase family protein	-
FORC2_RS02210	506370..508298	+	1929	WP_072084089.1	BglG family transcription antiterminator	-
FORC2_RS02215	508371..508676	-	306	WP_005162529.1	HigA family addiction module antidote protein	Antitoxin
FORC2_RS02220	508676..508954	-	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC2_RS02225	509034..511754	-	2721	WP_046694480.1	magnesium-translocating P-type ATPase	-
FORC2_RS02230	512261..513208	+	948	WP_046050170.1	trehalose operon repressor TreR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T53456 WP_005162526.1 NZ_CP011118:c508954-508676 [Yersinia enterocolitica]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T53456 NZ_CP011118:c508954-508676 [Yersinia enterocolitica]
ATGATAAAAAGTTGGAAACACAAAGGACTGCAACGCTTCTTTGAAACAGGTTCTGCTGCGGGCATTAATCCCCAGCATGT
TAAGCGAATTCGAGACCGCTTGCGATTTATCGACCTGGCGGAAACGATTGAGGATGTCAATGTTGCCGGATACAAGCTCC
ATCCGTTACAAGGTGAAAGAGAAGGGATCTGGTCAGTAACAGTAAGCGGAAATTGGCGCATTACATTCGAGTTTACCGCT
GGAGATGCGTTTATTCTTAACTACGAGGACTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10934.77 Da
• Isoelectric Point: 6.7158

>AT53456 WP_005162529.1 NZ_CP011118:c508676-508371 [Yersinia enterocolitica]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVACKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT53456 NZ_CP011118:c508676-508371 [Yersinia enterocolitica]
ATGAAAATGCATAATCCCCCACATCCAGGGGAAATGATTGAAGGAATCCTCGAAGATTTGGGGCTCGGGATAAGGGAGTT
TGCCAGAGCATTAAACGTGTCACCTTCAACGGTACAACGGCTGGTTGCCTGTAAGGCGGCAATTTCACCGGAAATGGCGG
TAAAACTGGCCGCGGTTCTGGGAAGTACCCCGGCAATGTGGCTCCGCCTCCAGGCCGCTTACGACCTGGGAAAAGCAGAA
GAGAATGTTGATGTATCCAATCTGACTAAACTGTACAAACCGGATCCAATATCGCTGCATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5