Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2052074..2052576 Replicon chromosome
Accession NZ_CP011113
Organism Escherichia coli strain RR1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SR35_RS11125 Protein ID WP_000767829.1
Coordinates 2052074..2052328 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SR35_RS11130 Protein ID WP_001259255.1
Coordinates 2052325..2052576 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR35_RS11100 2047089..2047316 - 228 WP_000234896.1 sulfurtransferase TusA -
SR35_RS11105 2047330..2048388 - 1059 WP_000492339.1 transport protein YeeE -
SR35_RS11110 2048567..2049925 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SR35_RS24905 2049915..2049977 - 63 WP_010723108.1 membrane protein YoeI -
SR35_RS11115 2050192..2051121 - 930 WP_000803351.1 LysR family transcriptional regulator -
SR35_RS11120 2051167..2051991 - 825 WP_000754737.1 SDR family oxidoreductase -
SR35_RS11125 2052074..2052328 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SR35_RS11130 2052325..2052576 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SR35_RS11135 2052859..2052909 + 51 WP_001364200.1 his operon leader peptide -
SR35_RS11145 2053055..2053954 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
SR35_RS11150 2053960..2055264 + 1305 WP_000009594.1 histidinol dehydrogenase -
SR35_RS11155 2055261..2056331 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SR35_RS11160 2056331..2057398 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53415 WP_000767829.1 NZ_CP011113:c2052328-2052074 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53415 NZ_CP011113:c2052328-2052074 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53415 WP_001259255.1 NZ_CP011113:c2052576-2052325 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53415 NZ_CP011113:c2052576-2052325 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References