Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 256077..256771 Replicon chromosome
Accession NZ_CP011113
Organism Escherichia coli strain RR1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag SR35_RS01275 Protein ID WP_001263489.1
Coordinates 256373..256771 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag SR35_RS01270 Protein ID WP_000554758.1
Coordinates 256077..256370 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR35_RS01245 251709..252206 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
SR35_RS01255 252430..254142 - 1713 Protein_228 flagellar biosynthesis protein FlhA -
SR35_RS01260 254114..254899 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
SR35_RS01265 254970..256025 + 1056 WP_001226164.1 DNA polymerase IV -
SR35_RS01270 256077..256370 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
SR35_RS01275 256373..256771 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
SR35_RS01280 256781..257233 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
SR35_RS01290 257551..257757 + 207 Protein_234 RtcB family protein -
SR35_RS01295 257753..258274 + 522 Protein_235 peptide chain release factor H -
SR35_RS01300 258331..259788 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
SR35_RS01310 260110..261090 - 981 WP_000019403.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T53401 WP_001263489.1 NZ_CP011113:256373-256771 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T53401 NZ_CP011113:256373-256771 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT53401 WP_000554758.1 NZ_CP011113:256077-256370 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT53401 NZ_CP011113:256077-256370 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References