53392

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6099515..6100209 Replicon chromosome
Accession NZ_CP011110
Organism Pseudomonas chlororaphis strain PCL1606

Toxin (Protein)


Gene name higB Uniprot ID A0A0D5Y7Q2
Locus tag PCL1606_RS27330 Protein ID WP_045886147.1
Coordinates 6099913..6100209 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D5Y6S9
Locus tag PCL1606_RS27325 Protein ID WP_045886145.1
Coordinates 6099515..6099916 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCL1606_RS27315 6094649..6097999 + 3351 WP_045886140.1 mechanosensitive channel MscK -
PCL1606_RS27320 6098048..6099511 + 1464 WP_045886142.1 YdiU family protein -
PCL1606_RS27325 6099515..6099916 - 402 WP_045886145.1 transcriptional regulator Antitoxin
PCL1606_RS27330 6099913..6100209 - 297 WP_045886147.1 type II toxin-antitoxin system HigB family toxin Toxin
PCL1606_RS27335 6100441..6100875 + 435 WP_044460807.1 thioredoxin TrxC -
PCL1606_RS27340 6100990..6101760 - 771 WP_044460806.1 ParA family protein -
PCL1606_RS27345 6102101..6102820 + 720 WP_045886149.1 beta-ketoacyl synthase chain length factor -
PCL1606_RS27350 6102796..6103611 + 816 WP_045886151.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
PCL1606_RS27355 6103586..6103846 + 261 WP_044460803.1 acyl carrier protein -
PCL1606_RS27360 6103856..6104110 + 255 WP_007926835.1 acyl carrier protein -
PCL1606_RS27365 6104107..6104652 + 546 WP_045886153.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Antitoxin HigA

(15-133)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11546.37 Da        Isoelectric Point: 10.3534

>T53392 WP_045886147.1 NZ_CP011110:c6100209-6099913 [Pseudomonas chlororaphis]
MHIITAKRIWEAKERWPQSASALDTWYRKIKGLNPSDFAAIKAVFPATDKVGAFHVFDVGGNKLRIIALVRYEVRRLYIR
HVLDHHEYDKGRWKEETI

Download         Length: 297 bp

>T53392 NZ_CP011110:c6100209-6099913 [Pseudomonas chlororaphis]
ATGCATATCATCACTGCCAAACGAATCTGGGAGGCCAAGGAGCGTTGGCCGCAATCGGCCAGTGCGCTGGATACCTGGTA
CAGAAAAATCAAAGGCCTCAACCCGTCCGACTTTGCAGCCATCAAGGCAGTGTTTCCTGCCACGGACAAAGTGGGGGCGT
TTCACGTTTTCGACGTTGGCGGCAACAAGTTACGCATCATCGCCCTGGTGCGTTATGAAGTACGACGGCTCTATATCAGG
CACGTACTGGACCATCATGAATACGACAAGGGCAGGTGGAAGGAGGAAACCATATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 15194.31 Da        Isoelectric Point: 4.6984

>AT53392 WP_045886145.1 NZ_CP011110:c6099916-6099515 [Pseudomonas chlororaphis]
MNVVVRQAAEHWRFVAPLLRKPKTEADYDELVRALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPVASGVDVL
RSFMREHGLTQSDLPGVGAQSVVSEILSGKRQLNLRQIRWLAERFKVSVETFI

Download         Length: 402 bp

>AT53392 NZ_CP011110:c6099916-6099515 [Pseudomonas chlororaphis]
ATGAACGTTGTGGTGAGGCAGGCGGCGGAGCATTGGCGTTTCGTGGCGCCGTTGCTGCGCAAACCCAAGACCGAGGCCGA
TTACGATGAATTGGTCCGGGCCCTGGATGAGCTGCTGGATCTCATGGGAGAGGACGAGAATCATCCGGTGACTTCACTGG
TGGACATCATCGGTGACTGGATCGAAGCCTATGACGAAGAGCATCGGCCAATGCCAGTGGCCAGCGGGGTCGATGTGTTG
CGCTCGTTCATGCGCGAACACGGCCTGACCCAGAGCGACTTGCCCGGCGTGGGCGCGCAGTCGGTGGTCTCGGAGATTCT
CAGCGGCAAGCGCCAGCTCAACCTGCGGCAGATCCGCTGGCTGGCCGAGCGCTTCAAGGTCTCGGTGGAAACCTTTATCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D5Y7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A0D5Y6S9

References