Detailed information of TA system TA053385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2479736..2480390
• Replicon: chromosome
• Accession: NZ_CP011110
• Organism: Pseudomonas chlororaphis strain PCL1606

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0C5EC26
• Locus tag: PCL1606_RS11465
• Protein ID: WP_044463940.1
• Coordinates: 2480040..2480390 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PCL1606_RS11460
• Protein ID: WP_044463941.1
• Coordinates: 2479736..2480050 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCL1606_RS11440	2475843..2476709	+	867	WP_009049796.1	sugar ABC transporter permease	-
PCL1606_RS11445	2476720..2477520	+	801	WP_044463944.1	carbohydrate ABC transporter permease	-
PCL1606_RS11450	2477531..2477803	+	273	WP_044463943.1	DUF2160 domain-containing protein	-
PCL1606_RS11455	2477854..2479596	+	1743	WP_045882349.1	ABC transporter substrate-binding protein	-
PCL1606_RS11460	2479736..2480050	-	315	WP_044463941.1	transcriptional regulator	Antitoxin
PCL1606_RS11465	2480040..2480390	-	351	WP_044463940.1	hypothetical protein	Toxin
PCL1606_RS11470	2480632..2482095	-	1464	WP_044463939.1	NADH-quinone oxidoreductase subunit NuoN	-
PCL1606_RS11475	2482103..2483635	-	1533	WP_044463938.1	NADH-quinone oxidoreductase subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13651.69 Da
• Isoelectric Point: 8.5210

>T53385 WP_044463940.1 NZ_CP011110:c2480390-2480040 [Pseudomonas chlororaphis]
MDALFIELPPFQRHRQGLLDDELFHSLQLELLKNPQAGDLIEGTGGLRKLRFVDERRHKGKRSGIRVIYYCWCAGAQFWL
FTLYAKNQQDDLAPHQKKLLKHLLNQEIEARTQHET

Nucleotide

• Length: 351 bp

>T53385 NZ_CP011110:c2480390-2480040 [Pseudomonas chlororaphis]
ATGGACGCCCTTTTTATCGAACTGCCCCCGTTCCAGCGCCATCGCCAGGGCCTCCTGGACGATGAGCTGTTTCACAGCCT
GCAGCTGGAGTTGCTGAAGAACCCGCAGGCCGGCGACCTCATCGAAGGCACCGGCGGCTTGCGCAAGCTGCGCTTTGTCG
ATGAACGCCGGCACAAGGGCAAGCGCAGCGGGATCAGGGTGATTTATTACTGCTGGTGCGCGGGCGCTCAGTTCTGGCTC
TTTACCCTTTACGCGAAGAACCAGCAGGACGACCTGGCACCGCACCAGAAGAAACTGCTCAAGCACCTGCTGAACCAGGA
AATCGAGGCGAGGACCCAACATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11922.82 Da
• Isoelectric Point: 10.5451

>AT53385 WP_044463941.1 NZ_CP011110:c2480050-2479736 [Pseudomonas chlororaphis]
MKRDIFSELVEGFDALAKEREGKITLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLATLA

Nucleotide

• Length: 315 bp

>AT53385 NZ_CP011110:c2480050-2479736 [Pseudomonas chlororaphis]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAGGAGCGCGAGGGCAAAATCACACTGCG
CACCCACAAGGTGAAGCTGAACGCCTTGCTGCCGATCACCGCCGAAGAAGTGCTGGCGGTGCGCGAACAACTGAACCTGT
CCCGCTCGGTGTTTGCCATGTACCTGCGCACCAACACCCGGACCCTGGAAAACTGGGAACAAGGGCGCGCAAAACCCAAC
GCTCAGGCCACGACCCTGATCCGCCTGGTACAGCGCTTTCCCGATACTGTGGAGCGGCTGGCGACACTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0C5EC26