Detailed information of TA system TA053309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4810631..4811349
• Replicon: chromosome
• Accession: NZ_CP011077
• Organism: Klebsiella michiganensis strain RC10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: VW41_RS22380
• Protein ID: WP_045784266.1
• Coordinates: 4810631..4810942 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VW41_RS22385
• Protein ID: WP_008458601.1
• Coordinates: 4810939..4811349 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VW41_RS22365	4805810..4806880	-	1071	WP_045784263.1	SIS domain-containing protein	-
VW41_RS22370	4807044..4808090	-	1047	WP_045784264.1	LacI family transcriptional regulator	-
VW41_RS22375	4808275..4810488	+	2214	WP_045784265.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
VW41_RS22380	4810631..4810942	+	312	WP_045784266.1	type II toxin-antitoxin system HigB family toxin	Toxin
VW41_RS22385	4810939..4811349	+	411	WP_008458601.1	helix-turn-helix domain-containing protein	Antitoxin
VW41_RS22390	4811375..4811620	-	246	WP_045784267.1	sulfurtransferase TusA	-
VW41_RS22395	4811795..4812460	+	666	WP_045784268.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
VW41_RS22400	4812533..4813090	+	558	WP_045784269.1	DcrB family lipoprotein	-
VW41_RS22405	4813222..4814271	+	1050	WP_045784270.1	AI-2E family transporter	-
VW41_RS22410	4814278..4814919	-	642	WP_045784746.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12305.53 Da
• Isoelectric Point: 10.6532

>T53309 WP_045784266.1 NZ_CP011077:4810631-4810942 [Klebsiella michiganensis]
MHLITLKALVEAGQKFPRHKQELQILGKVLEKGFFPTPDSLKRVYPTLDNFKYLDKHYVINIAGNELRLIALIFFTSQKF
YIRHVMTHSEYMRFTEKHRGKKR

Nucleotide

• Length: 312 bp

>T53309 NZ_CP011077:4810631-4810942 [Klebsiella michiganensis]
ATGCATCTGATTACGCTTAAGGCGCTTGTGGAAGCCGGTCAAAAATTTCCCAGACATAAGCAGGAACTGCAAATCCTTGG
CAAAGTGCTGGAAAAAGGCTTCTTCCCAACACCGGACTCACTTAAGCGGGTTTATCCCACGCTGGATAACTTTAAATATC
TCGATAAACATTACGTCATCAACATAGCCGGAAATGAACTCAGGCTGATTGCACTTATCTTTTTTACCAGCCAGAAGTTC
TACATCCGCCACGTCATGACGCACAGCGAATACATGCGATTTACTGAAAAACACCGGGGAAAGAAGCGATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15144.52 Da
• Isoelectric Point: 5.6165

>AT53309 WP_008458601.1 NZ_CP011077:4810939-4811349 [Klebsiella michiganensis]
MIADALKAAEALKTAVPLLAGSTCEKDYLAALELVEYLLINDPESPLVEILSSKIQAWENAQPDVQAFREEMQAIPAGIA
ILKTLMEHHHLKQSDFQQEIGSKSMVSRILSGERQLTTDHIRKLSRRFGISPALFF

Nucleotide

• Length: 411 bp

>AT53309 NZ_CP011077:4810939-4811349 [Klebsiella michiganensis]
ATGATTGCGGATGCACTGAAGGCCGCCGAAGCATTAAAAACAGCGGTTCCGCTGCTGGCAGGCAGCACCTGCGAAAAAGA
TTATCTGGCTGCACTGGAGCTGGTCGAATATCTGCTCATCAATGATCCAGAAAGTCCGCTGGTTGAGATTCTCAGTAGTA
AGATCCAGGCCTGGGAAAACGCGCAGCCGGACGTGCAGGCGTTCCGCGAGGAAATGCAGGCCATACCTGCCGGGATAGCC
ATACTGAAAACCCTGATGGAGCATCACCATCTAAAACAGAGCGATTTTCAACAAGAGATTGGCAGCAAGTCGATGGTCTC
CCGCATTCTCAGCGGTGAACGCCAGCTAACCACCGACCACATCCGCAAACTGTCCCGGCGATTTGGCATATCACCCGCTC
TCTTTTTCTGA