Detailed information of TA system TA053286

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29637..30058
• Replicon: plasmid pSJ_94
• Accession: NZ_CP011064
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: Y979_RS26255
• Protein ID: WP_001302184.1
• Coordinates: 29900..30058 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29637..29835 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS26235	25333..26727	+	1395	WP_001037799.1	carbohydrate porin	-
Y979_RS30930	26973..27077	-	105	Protein_28	thiosulfate reductase cytochrome B subunit	-
Y979_RS26245	27134..27827	+	694	Protein_29	IS1 family transposase	-
Y979_RS26250	27843..28466	+	624	WP_000912556.1	potassium channel family protein	-
Y979_RS29365	28551..28802	+	252	Protein_31	iron-containing alcohol dehydrogenase	-
Y979_RS29370	29091..29408	-	318	Protein_32	IS110 family transposase	-
Y979_RS30295	29563..29668	+	106	Protein_33	plasmid SOS inhibition protein A	-
-	29637..29835	+	199	NuclAT_0	-	Antitoxin
-	29637..29835	+	199	NuclAT_0	-	Antitoxin
-	29637..29835	+	199	NuclAT_0	-	Antitoxin
-	29637..29835	+	199	NuclAT_0	-	Antitoxin
Y979_RS26255	29900..30058	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
Y979_RS31550	30726..30932	+	207	WP_000275854.1	hypothetical protein	-
Y979_RS26270	30957..31244	+	288	WP_000107242.1	hypothetical protein	-
Y979_RS26275	31363..32184	+	822	WP_001234465.1	DUF945 domain-containing protein	-
Y979_RS26280	32480..33052	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
Y979_RS26285	33422..33805	+	384	WP_001063020.1	relaxosome protein TraM	-
Y979_RS26290	33996..34643	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
Y979_RS29375	34779..34994	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..94712	94712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T53286 WP_001302184.1 NZ_CP011064:29900-30058 [Escherichia coli str. Sanji]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T53286 NZ_CP011064:29900-30058 [Escherichia coli str. Sanji]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT53286 NZ_CP011064:29637-29835 [Escherichia coli str. Sanji]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin