Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2175404..2175906 Replicon chromosome
Accession NZ_CP011061
Organism Escherichia coli str. Sanji

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag Y979_RS10860 Protein ID WP_000767829.1
Coordinates 2175404..2175658 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag Y979_RS10865 Protein ID WP_001259255.1
Coordinates 2175655..2175906 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y979_RS10835 2170419..2170646 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
Y979_RS10840 2170660..2171718 - 1059 WP_000492321.1 transport protein YeeE -
Y979_RS10845 2171897..2173255 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
Y979_RS30605 2173245..2173307 - 63 WP_010723108.1 membrane protein YoeI -
Y979_RS10850 2173522..2174451 - 930 WP_000803351.1 LysR family transcriptional regulator -
Y979_RS10855 2174497..2175321 - 825 WP_000754749.1 SDR family oxidoreductase -
Y979_RS10860 2175404..2175658 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
Y979_RS10865 2175655..2175906 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
Y979_RS28040 2176190..2176240 + 51 WP_001364200.1 his operon leader peptide -
Y979_RS10870 2176386..2177285 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
Y979_RS10875 2177291..2178595 + 1305 WP_001446927.1 histidinol dehydrogenase -
Y979_RS10880 2178592..2179662 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
Y979_RS10885 2179662..2180729 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53273 WP_000767829.1 NZ_CP011061:c2175658-2175404 [Escherichia coli str. Sanji]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53273 NZ_CP011061:c2175658-2175404 [Escherichia coli str. Sanji]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53273 WP_001259255.1 NZ_CP011061:c2175906-2175655 [Escherichia coli str. Sanji]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53273 NZ_CP011061:c2175906-2175655 [Escherichia coli str. Sanji]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References