Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 285349..286043 Replicon chromosome
Accession NZ_CP011061
Organism Escherichia coli str. Sanji

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag Y979_RS01305 Protein ID WP_001263489.1
Coordinates 285645..286043 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag Y979_RS01300 Protein ID WP_000554758.1
Coordinates 285349..285642 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y979_RS01280 280981..281478 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
Y979_RS01285 281702..283414 - 1713 Protein_246 flagellar biosynthesis protein FlhA -
Y979_RS01290 283386..284171 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
Y979_RS01295 284242..285297 + 1056 WP_001226164.1 DNA polymerase IV -
Y979_RS01300 285349..285642 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
Y979_RS01305 285645..286043 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
Y979_RS01310 286053..286505 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
Y979_RS01315 286823..287029 + 207 Protein_252 RtcB family protein -
Y979_RS01320 287025..287546 + 522 Protein_253 peptide chain release factor H -
Y979_RS01325 287603..289060 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
Y979_RS01330 289321..289779 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 290375..290455 + 81 NuclAT_11 - -
- 290375..290455 + 81 NuclAT_11 - -
- 290375..290455 + 81 NuclAT_11 - -
- 290375..290455 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T53259 WP_001263489.1 NZ_CP011061:285645-286043 [Escherichia coli str. Sanji]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T53259 NZ_CP011061:285645-286043 [Escherichia coli str. Sanji]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT53259 WP_000554758.1 NZ_CP011061:285349-285642 [Escherichia coli str. Sanji]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT53259 NZ_CP011061:285349-285642 [Escherichia coli str. Sanji]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References