Detailed information of TA system TA053225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3263963..3264528
• Replicon: chromosome
• Accession: NZ_CP011047
• Organism: Cronobacter sakazakii strain ATCC 29544

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7V7RF17
• Locus tag: CSK29544_RS15580
• Protein ID: WP_029039105.1
• Coordinates: 3264250..3264528 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CSK29544_RS15575
• Protein ID: WP_004388462.1
• Coordinates: 3263963..3264250 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSK29544_RS15560	3259559..3260425	+	867	WP_007888776.1	ABC transporter permease	-
CSK29544_RS15565	3260455..3261513	-	1059	WP_007888777.1	YncE family protein	-
CSK29544_RS15570	3261791..3263908	+	2118	WP_029039106.1	TonB-dependent receptor	-
CSK29544_RS15575	3263963..3264250	-	288	WP_004388462.1	HigA family addiction module antidote protein	Antitoxin
CSK29544_RS15580	3264250..3264528	-	279	WP_029039105.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSK29544_RS15585	3264828..3266432	+	1605	WP_007888783.1	alpha-amylase family protein	-
CSK29544_RS15590	3266434..3267420	-	987	WP_007888785.1	SDR family oxidoreductase	-
CSK29544_RS15595	3267528..3269465	-	1938	WP_007888791.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10628.13 Da
• Isoelectric Point: 10.4224

>T53225 WP_029039105.1 NZ_CP011047:c3264528-3264250 [Cronobacter sakazakii]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGLWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T53225 NZ_CP011047:c3264528-3264250 [Cronobacter sakazakii]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGATTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTCTATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTTATCGTCAACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10428.03 Da
• Isoelectric Point: 8.7791

>AT53225 WP_004388462.1 NZ_CP011047:c3264250-3263963 [Cronobacter sakazakii]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT53225 NZ_CP011047:c3264250-3263963 [Cronobacter sakazakii]
ATGGCAACGATGTTTAACCCACCGCACCCGGGCAGGCTGGTGCAGGAGTCAATGGAAGCGCTCGGCCTCAGCGCCCGTGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCGACGGTGCAGCGTCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAGAATGAATACGATCTCTTTCAGGCG
CGCCAGTCTGTTGATTTCTCGCACCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: A0A7V7RF17