Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2496153..2496800 Replicon chromosome
Accession NZ_CP011047
Organism Cronobacter sakazakii strain ATCC 29544

Toxin (Protein)


Gene name higB Uniprot ID A0A2S9UA56
Locus tag CSK29544_RS11940 Protein ID WP_004387788.1
Coordinates 2496153..2496500 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V5TYW5
Locus tag CSK29544_RS11945 Protein ID WP_007776551.1
Coordinates 2496501..2496800 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSK29544_RS11915 2491384..2492394 + 1011 WP_004387466.1 galactose/methyl galactoside ABC transporter permease MglC -
CSK29544_RS11920 2492955..2493668 - 714 WP_004387784.1 outer membrane permeability protein SanA -
CSK29544_RS11925 2493793..2494677 - 885 WP_029039355.1 cytidine deaminase -
CSK29544_RS11930 2494807..2495502 - 696 WP_007899537.1 CidB/LrgB family autolysis modulator -
CSK29544_RS11935 2495499..2495900 - 402 WP_007869367.1 CidA/LrgA family protein -
CSK29544_RS11940 2496153..2496500 + 348 WP_004387788.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CSK29544_RS11945 2496501..2496800 + 300 WP_007776551.1 XRE family transcriptional regulator Antitoxin
CSK29544_RS11950 2496873..2497805 + 933 WP_007899538.1 tRNA dihydrouridine(16) synthase DusC -
CSK29544_RS11955 2497808..2498731 - 924 WP_007899542.1 LysR family transcriptional regulator -
CSK29544_RS11960 2498804..2499763 + 960 WP_007899546.1 MBL fold metallo-hydrolase -
CSK29544_RS22915 2499800..2499883 - 84 WP_000691708.1 protein YohP -
CSK29544_RS11965 2500056..2500829 + 774 WP_007899548.1 SDR family oxidoreductase -
CSK29544_RS11970 2500826..2501392 - 567 WP_007899550.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-83)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13457.72 Da        Isoelectric Point: 10.2057

>T53215 WP_004387788.1 NZ_CP011047:2496153-2496500 [Cronobacter sakazakii]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGRPYADTLKGSRHAGMKELRVQHAGRPIRAFYAFDPHRYAI
VLCAAQKKGDEKRFYRVMLRVADKLFSHYLNHREV

Download         Length: 348 bp

>T53215 NZ_CP011047:2496153-2496500 [Cronobacter sakazakii]
ATGTGGACTATTTACCTTACTCACGAGTTTGAATGCTGGCTCGCGAGACAGCCGCAGCCGTTGCAGGAAGATGTGCTGGC
CGCGCTGGGGCTTTTAAAAATCAAAGGTCCGCACCTCGGGCGTCCGTATGCCGATACGCTCAAAGGCTCGCGTCACGCGG
GTATGAAGGAATTACGGGTTCAGCACGCCGGGCGGCCCATACGCGCCTTTTACGCTTTTGATCCGCACCGCTATGCGATT
GTGCTGTGCGCGGCACAGAAAAAGGGCGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACCGGGAGGTATAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11035.67 Da        Isoelectric Point: 5.1667

>AT53215 WP_007776551.1 NZ_CP011047:2496501-2496800 [Cronobacter sakazakii]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Download         Length: 300 bp

>AT53215 NZ_CP011047:2496501-2496800 [Cronobacter sakazakii]
ATGAAGACGTTTGATGAAGTGCTGGCGGCGCAGACCCCGGAGGCCCGCGCACGCATCGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCACCTGTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGTATCGCCC
AGCCGTCAGTCGCGGCTATCGAACAGCGCGGCAACGAGATGAAAATCTCAACGCTTAAGCGCTATGTCGAAGCCCTGGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9UA56

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UA72

References