Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4715335..4715927 Replicon chromosome
Accession NZ_CP011020
Organism Pseudomonas chlororaphis strain UFB2 isolate Soil

Toxin (Protein)


Gene name higB Uniprot ID A0A0G3GLH4
Locus tag VM99_RS20680 Protein ID WP_047738402.1
Coordinates 4715335..4715613 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A0D0PR32
Locus tag VM99_RS20685 Protein ID WP_042728178.1
Coordinates 4715622..4715927 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VM99_RS20665 4711185..4711700 + 516 WP_042728200.1 sigma-70 family RNA polymerase sigma factor -
VM99_RS20670 4711687..4712667 + 981 WP_047738399.1 DUF4880 domain-containing protein -
VM99_RS20675 4712789..4715161 + 2373 WP_047738400.1 TonB-dependent siderophore receptor -
VM99_RS20680 4715335..4715613 + 279 WP_047738402.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VM99_RS20685 4715622..4715927 + 306 WP_042728178.1 HigA family addiction module antidote protein Antitoxin
VM99_RS20690 4715947..4717428 - 1482 WP_047738404.1 hypothetical protein -
VM99_RS20695 4717649..4718194 + 546 WP_047738406.1 RNA 2'-phosphotransferase -
VM99_RS20700 4718252..4718593 - 342 WP_042728175.1 hypothetical protein -
VM99_RS20705 4719329..4720177 - 849 WP_047738408.1 phosphatase PAP2 family protein -
VM99_RS20710 4720202..4720894 - 693 WP_047738409.1 histidine phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4684467..4723774 39307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-97)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10381.92 Da        Isoelectric Point: 7.7522

>T53199 WP_047738402.1 NZ_CP011020:4715335-4715613 [Pseudomonas chlororaphis]
MIRSFKCADIQALFETGKARRWAAILSVAERKLGMLNAAVELNDLCSPPGNRLEPLQGDRQGQHSIRINGQYRICFVWGI
NGPEQVEIVDYH

Download         Length: 279 bp

>T53199 NZ_CP011020:4715335-4715613 [Pseudomonas chlororaphis]
ATGATCCGAAGCTTCAAATGCGCCGACATCCAAGCCCTTTTTGAAACCGGCAAGGCCCGGCGCTGGGCCGCTATCCTTTC
AGTTGCGGAACGAAAGCTGGGGATGCTCAACGCAGCGGTCGAACTCAATGACCTCTGCTCCCCACCGGGAAACAGGCTGG
AGCCGCTGCAGGGCGACAGACAGGGTCAGCACAGCATTCGGATCAATGGTCAGTACCGAATCTGTTTTGTCTGGGGCATC
AATGGCCCCGAACAGGTAGAAATCGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 7.2768

>AT53199 WP_042728178.1 NZ_CP011020:4715622-4715927 [Pseudomonas chlororaphis]
MFKNGMRPVHPGEILKEEYLEPLGLSAAALARALHVSAPTINDIVLKRRSISADVALRLAAALDTSAQFWLNLQIAYDLR
TAEIAKGEQIYREVEKVPHCA

Download         Length: 306 bp

>AT53199 NZ_CP011020:4715622-4715927 [Pseudomonas chlororaphis]
ATGTTTAAGAACGGCATGAGGCCTGTACATCCCGGGGAAATTCTCAAAGAAGAATACCTGGAACCACTTGGTCTTTCGGC
AGCCGCGCTTGCAAGGGCTTTGCACGTTTCGGCTCCCACGATAAATGACATTGTGTTGAAGCGTCGCAGCATCAGCGCAG
ATGTTGCCTTGCGCTTGGCAGCCGCGTTGGATACGTCAGCGCAATTTTGGCTGAACCTGCAGATTGCCTATGATTTACGT
ACAGCCGAGATTGCTAAAGGTGAGCAGATCTACCGGGAAGTGGAGAAGGTTCCTCACTGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G3GLH4

Antitoxin

Source ID Structure
AlphaFold DB A0A0D0PR32

References