Detailed information of TA system TA053198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4456315..4456970
• Replicon: chromosome
• Accession: NZ_CP011020
• Organism: Pseudomonas chlororaphis strain UFB2 isolate Soil

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0D0PKE3
• Locus tag: VM99_RS19555
• Protein ID: WP_042728258.1
• Coordinates: 4456626..4456970 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VM99_RS19550
• Protein ID: WP_042728259.1
• Coordinates: 4456315..4456629 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VM99_RS19530	4452406..4453323	+	918	WP_047738240.1	LysR family transcriptional regulator	-
VM99_RS19535	4453438..4454451	+	1014	WP_047739532.1	diguanylate cyclase	-
VM99_RS19540	4454518..4455261	+	744	WP_047738242.1	DUF72 domain-containing protein	-
VM99_RS19545	4455400..4456104	+	705	WP_042728260.1	hypothetical protein	-
VM99_RS19550	4456315..4456629	-	315	WP_042728259.1	helix-turn-helix domain-containing protein	Antitoxin
VM99_RS19555	4456626..4456970	-	345	WP_042728258.1	hypothetical protein	Toxin
VM99_RS19560	4457109..4457996	-	888	WP_025211928.1	heme o synthase	-
VM99_RS19565	4458008..4458343	-	336	WP_025211927.1	cytochrome o ubiquinol oxidase subunit IV	-
VM99_RS19570	4458344..4458970	-	627	WP_042728257.1	cytochrome o ubiquinol oxidase subunit III	-
VM99_RS19575	4458974..4461004	-	2031	WP_042728256.1	cytochrome o ubiquinol oxidase subunit I	-
VM99_RS19580	4461008..4461955	-	948	WP_042728255.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13598.59 Da
• Isoelectric Point: 9.2897

>T53198 WP_042728258.1 NZ_CP011020:c4456970-4456626 [Pseudomonas chlororaphis]
MHTLFFETTAFTATVGHYLTDDEYRLLQSYLLQHPTAGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDLQFWM
FAIYDKDELENLTREQEKSLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T53198 NZ_CP011020:c4456970-4456626 [Pseudomonas chlororaphis]
ATGCATACCTTATTTTTTGAAACGACTGCGTTCACCGCCACGGTGGGACATTACCTGACTGACGATGAATATCGTTTGCT
CCAGTCCTATCTGCTGCAGCACCCAACGGCCGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAGCGTCGTGGCAAGGGGAAGCGAGGTGGTTTGCGAGTCATTTACTATTGGCTCATGAATGATCTTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCCGCGAGCAAGAGAAGTCGCTCAAGCGCGCCATTGAGGCTGA
GTTGAAAGTGCGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11813.76 Da
• Isoelectric Point: 9.9445

>AT53198 WP_042728259.1 NZ_CP011020:c4456629-4456315 [Pseudomonas chlororaphis]
MKKRDLFAEMMQGIEEMAAHREGKLTLRQVSIEDKPAPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSRP
NAQAALLIRLVEKFPDMVERLAAV

Nucleotide

• Length: 315 bp

>AT53198 NZ_CP011020:c4456629-4456315 [Pseudomonas chlororaphis]
ATGAAAAAACGTGATCTGTTTGCCGAGATGATGCAGGGCATCGAGGAAATGGCCGCTCACCGGGAGGGCAAGCTGACCCT
TCGCCAGGTATCGATCGAAGATAAACCAGCGCCCGAGGTATCGGCCCAGGAAATTGTGGCGTTGCGTGACAAGCTCCACA
TGTCCCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCTGGCACGCTCAGGAACTGGGAGCAGGAAAAGTCCAGGCCC
AATGCCCAGGCGGCTCTGTTGATCAGGTTGGTCGAGAAGTTTCCAGACATGGTCGAGCGGCTCGCAGCTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0D0PKE3