Detailed information of TA system TA053192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3954406..3955205
• Replicon: chromosome
• Accession: NZ_CP011018
• Organism: Escherichia coli strain CI5

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: VK74_RS19650
• Protein ID: WP_000347253.1
• Coordinates: 3954406..3954870 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: VK74_RS19655
• Protein ID: WP_024251361.1
• Coordinates: 3954870..3955205 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK74_RS19620	3949407..3949841	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
VK74_RS19625	3949859..3950737	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
VK74_RS19630	3950727..3951506	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
VK74_RS19635	3951517..3951990	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
VK74_RS19640	3952013..3953293	-	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
VK74_RS19645	3953542..3954351	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
VK74_RS19650	3954406..3954870	-	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
VK74_RS19655	3954870..3955205	-	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
VK74_RS19660	3955354..3956925	-	1572	WP_001273766.1	galactarate dehydratase	-
VK74_RS19665	3957300..3958634	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
VK74_RS19670	3958650..3959420	+	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T53192 WP_000347253.1 NZ_CP011018:c3954870-3954406 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T53192 NZ_CP011018:c3954870-3954406 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT53192 WP_024251361.1 NZ_CP011018:c3955205-3954870 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT53192 NZ_CP011018:c3955205-3954870 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA