53187

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2329435..2329976 Replicon chromosome
Accession NZ_CP011018
Organism Escherichia coli strain CI5

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag VK74_RS11725 Protein ID WP_000615976.1
Coordinates 2329698..2329976 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag VK74_RS11720 Protein ID WP_000729704.1
Coordinates 2329435..2329695 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK74_RS11695 2324471..2325610 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
VK74_RS11700 2325594..2327687 + 2094 WP_001140827.1 flagellar biosynthesis protein FlhA -
VK74_RS11705 2327792..2328070 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
VK74_RS11710 2328063..2328419 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
VK74_RS11715 2328476..2329249 - 774 WP_000543895.1 C40 family peptidase -
VK74_RS11720 2329435..2329695 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
VK74_RS11725 2329698..2329976 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
VK74_RS11730 2330132..2330872 + 741 WP_001225679.1 murein L,D-transpeptidase -
VK74_RS11735 2330843..2331610 - 768 WP_000333380.1 class II glutamine amidotransferase -
VK74_RS11740 2331816..2332394 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / hsiC1/vipB / hsiB1/vipA 2328063..2365249 37186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T53187 WP_000615976.1 NZ_CP011018:2329698-2329976 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T53187 NZ_CP011018:2329698-2329976 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT53187 WP_000729704.1 NZ_CP011018:2329435-2329695 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT53187 NZ_CP011018:2329435-2329695 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References